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Western Blot (WB) (Western blot analysis of TCEAL1 expression in HepG2 (A), Hela (B) whole cell lysates.)

Rabbit TCEAL1 Polyclonal Antibody | anti-TCEAL1 antibody

Anti-TCEAL1 Antibody

Gene Names
TCEAL1; p21; SIIR; WEX9; pp21
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
TCEAL1; Polyclonal Antibody; Anti-TCEAL1 Antibody; SIIR; Transcription elongation factor A protein-like 1; TCEA-like protein 1; Nuclear phosphoprotein p21/SIIR; Transcription elongation factor S-II protein-like 1; anti-TCEAL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of TCEAL1 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
157
Applicable Applications for anti-TCEAL1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TCEAL1. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of TCEAL1 expression in HepG2 (A), Hela (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of TCEAL1 expression in HepG2 (A), Hela (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of TCEAL1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of TCEAL1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunofluorescence (IF)

(Immunofluorescent analysis of TCEAL1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Immunofluorescence (IF) (Immunofluorescent analysis of TCEAL1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-TCEAL1 antibody
Rabbit polyclonal antibody to TCEAL1

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Transcription elongation factor A protein-like 1
NCBI Official Synonym Full Names
transcription elongation factor A like 1
NCBI Official Symbol
TCEAL1
NCBI Official Synonym Symbols
p21; SIIR; WEX9; pp21
NCBI Protein Information
transcription elongation factor A protein-like 1
UniProt Protein Name
Transcription elongation factor A protein-like 1
UniProt Gene Name
TCEAL1
UniProt Synonym Gene Names
SIIR; TCEA-like protein 1
UniProt Entry Name
TCAL1_HUMAN

NCBI Description

This gene encodes a member of the transcription elongation factor A (SII)-like (TCEAL) gene family. Members of this family may function as nuclear phosphoproteins that modulate transcription in a promoter context-dependent manner. The encoded protein is similar to transcription elongation factor A/transcription factor SII and contains a zinc finger-like motif as well as a sequence related to the transcription factor SII Pol II-binding region. It may exert its effects via protein-protein interactions with other transcriptional regulators rather than via direct binding of DNA. Multiple family members are located on the X chromosome. Alternative splicing results in multiple transcript variants encoding a single isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

May be involved in transcriptional regulation. Modulates various viral and cellular promoters in a promoter context-dependent manner. For example, transcription from the FOS promoter is increased, while Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter activity is repressed. Does not bind DNA directly.

Research Articles on TCEAL1

Similar Products

Product Notes

The TCEAL1 tceal1 (Catalog #AAA8242854) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TCEAL1 Antibody reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's TCEAL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the TCEAL1 tceal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCEAL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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