Rabbit anti-Human TCEA2 Polyclonal Antibody | anti-TCEA2 antibody

TCEA2, ID (TCEA2, Transcription elongation factor A protein 2, Testis-specific S-II, Transcription elongation factor S-II protein 2, Transcription elongation factor TFIIS.l) (Biotin)

Cat. Num. AAA6354339

• Gene Names: TCEA2; TFIIS
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TCEA2; Polyclonal Antibody; ID (TCEA2; Transcription elongation factor A protein 2; Testis-specific S-II; Transcription elongation factor S-II protein 2; Transcription elongation factor TFIIS.l) (Biotin); Transcription elongation factor TFIIS.l; anti-TCEA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TCEA2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TCEA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-115 amino acids from the Central region of human TCEA2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TCEA2 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the TCEA2 antibody detected the TCEA2 protein (arrow).)

Related Product Information for anti-TCEA2 antibody

The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TCEA2 antibody

Antibodies; Transcription Factors

References

Kuraoka, I., et al. DNA Repair (Amst.) 6(6):841-851(2007). Palangat, M., et al. Proc. Natl. Acad. Sci. U.S.A. 102(42):15036-15041(2005). Stelzl, U., et al. Cell 122(6):957-968(2005). Nakata, A., et al. Genes Cells 9(11):1125-1135(2004). Tamura, K., et al. Biochem. Biophys. Res. Commun. 309(1):189-195(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4507385
• NCBI GeneID: 6919
• NCBI Accession #: NP_003186.1; NP_942016.1
• NCBI GenBank Nucleotide #: NM_003195.4
• UniProt Accession #: Q15560; Q8TD37; Q8TD38; B3KNM1
• Molecular Weight: 30,569 Da
• NCBI Official Full Name: transcription elongation factor A protein 2 isoform a
• NCBI Official Synonym Full Names: transcription elongation factor A (SII), 2
• NCBI Official Symbol: TCEA2
• NCBI Official Synonym Symbols: TFIIS
• NCBI Protein Information: transcription elongation factor A protein 2; testis-specific S-II; transcription elongation factor S-II protein 2; transcription elongation factor TFIIS.1; transcription elongation factor TFIIS.l
• UniProt Protein Name: Transcription elongation factor A protein 2
• Protein Family: Tuliposide A-converting enzyme
• UniProt Gene Name: TCEA2
• UniProt Entry Name: TCEA2_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TCEA2: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Belongs to the TFS-II family.

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nucleoplasm; transcription elongation factor complex; centrosome

Molecular Function: protein binding; DNA binding; zinc ion binding

Biological Process: RNA elongation; positive regulation of transcription from RNA polymerase II promoter; regulation of RNA elongation