Rabbit anti-Human TBX6 Polyclonal Antibody | anti-TBX6 antibody

TBX6, CT (TBX6, T-box transcription factor TBX6) (PE)

Cat. Num. AAA6354248

• Gene Names: TBX6; SCDO5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: TBX6; Polyclonal Antibody; CT (TBX6; T-box transcription factor TBX6) (PE); T-box transcription factor TBX6; anti-TBX6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TBX6 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: TBX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-180 amino acids from the C-terminal region of human TBX6.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TBX6 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow).)

Flow Cytometry (FC/FACS)

(TBX6 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TBX6 antibody

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures.

Product Categories/Family for anti-TBX6 antibody

Antibodies; Transcription Factors

References

Fei, Q., et al. Spine 35(9):983-988(2010). Ghebranious, N., et al. J. Bone Miner. Res. 23(10):1576-1583(2008). Farin, H.F., et al. J. Biol. Chem. 282(35):25748-25759(2007). Papapetrou, C., et al. Genomics 55(2):238-241(1999). Yi, C.H., et al. Genomics 55(1):10-20(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 18201900
• NCBI GeneID: 6911
• NCBI Accession #: NP_004599
• NCBI GenBank Nucleotide #: NM_004608
• UniProt Accession #: O95947
• Molecular Weight: 47kDa
• NCBI Official Full Name: T-box transcription factor TBX6
• NCBI Official Synonym Full Names: T-box 6
• NCBI Official Symbol: TBX6
• NCBI Official Synonym Symbols: SCDO5
• NCBI Protein Information: T-box transcription factor TBX6
• UniProt Protein Name: T-box transcription factor TBX6
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX6
• UniProt Synonym Gene Names: T-box protein 6
• UniProt Entry Name: TBX6_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures. [provided by RefSeq, Aug 2008]

Uniprot Description

TBX6: T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. Acts in part by down-regulating, a specific enhancer (N1) of SOX2, to inhibit neural development. Seems to play also an essential role in left/right axis determination and acts through effects on Notch signaling around the node as well as through an effect on the morphology and motility of the nodal cilia.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: negative regulation of neuron maturation; anatomical structure morphogenesis; transcription, DNA-dependent; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; cell fate specification; mesoderm development; negative regulation of transcription from RNA polymerase II promoter; somite rostral/caudal axis specification

Disease: Deafness, Autosomal Recessive 67