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Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SMAD2 protein using SMAD2 antibody)

Mouse SMAD2 Monoclonal Antibody | anti-SMAD2 antibody

Mouse monoclonal SMAD2 antibody

Applications
Immunofluorescence, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
SMAD2; Monoclonal Antibody; Mouse monoclonal SMAD2 antibody; SMAD2 antibody; anti-SMAD2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2C10
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
467
Applicable Applications for anti-SMAD2 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100
WB: 1:2000
Immunogen
SMAD2 antibody was raised in mouse using a full length recombinant protein of human SMAD2 (NP_005892) produced in HEK293T cells, as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SMAD2 protein using SMAD2 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant SMAD2 protein using SMAD2 antibody)
Product Categories/Family for anti-SMAD2 antibody

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
48,956 Da
NCBI Official Full Name
Smad2
UniProt Protein Name
Mothers against decapentaplegic homolog 2
UniProt Gene Name
SMAD2
UniProt Synonym Gene Names
MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2

Uniprot Description

SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: DNA binding; double-stranded DNA binding; phosphatase binding; protein binding; SMAD binding; transcription activator binding; transcription factor activity; transcription factor binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin protein ligase binding

Biological Process: activin receptor signaling pathway; anterior/posterior pattern formation; cell fate commitment; common-partner SMAD protein phosphorylation; gastrulation; mesoderm formation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; palate development; paraxial mesoderm morphogenesis; positive regulation of BMP signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; primary microRNA processing; regulation of binding; regulation of transforming growth factor beta receptor signaling pathway; SMAD protein complex assembly; somatic stem cell maintenance; transforming growth factor beta receptor signaling pathway; zygotic determination of dorsal/ventral axis

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Product Notes

The SMAD2 smad2 (Catalog #AAA5307342) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SMAD2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the SMAD2 smad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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