Western Blot (WB)
(Western blot (WB) analysis of TBK1 polyclonal antibody at 1:500 dilutionLaneA: The Testis tissue lysate of MouseLaneB: The Testis tissue lysate of RatLaneC: U-87MG whole cell lysateLaneD: A549 whole cell lysate)
Rabbit TBK1 Polyclonal Antibody | anti-TBK1 antibody
TBK1 polyclonal antibody
Gene Names
TBK1; NAK; T2K
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
TBK1; Polyclonal Antibody; TBK1 polyclonal antibody; Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1; NAK; anti-TBK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
TBK1 polyclonal antibody detects endogenous levels of TBK1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TBK1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:200
IHC: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human TBK1
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of TBK1 polyclonal antibody at 1:500 dilutionLaneA: The Testis tissue lysate of MouseLaneB: The Testis tissue lysate of RatLaneC: U-87MG whole cell lysateLaneD: A549 whole cell lysate)
Western Blot (WB)
(Western blot (WB) analysis of TBK1 polyclonal antibody at 1:500 dilutionLaneA: The Testis tissue lysate of MouseLaneB: The Testis tissue lysate of RatLaneC: U-87MG whole cell lysateLaneD: A549 whole cell lysate)
Related Product Information for anti-TBK1 antibody
The transcription factor NFkappaB is retained in the cytoplasm in an inactive form by the inhibitory protein IkappaB. Activation of NFkappaB requires that IkappaB be phosphorylated on specific serine residues, which results in targeted degradation of IkappaB. IkappaB kinase alpha (IKKalpha), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkappaB-alpha on the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKalpha, IKKbeta and IKKgamma (also designated NEMO), and each appear to make essential contributions to IkappaB phosphorylation. TANK binding kinase (TBK1), also designated T2K, is a novel IKK-related kinase that has been identified in murine and human tissues. TBK1 was shown to complex with TRAF2 and TANK in the NFkappaB activation pathway. TBK1 shares homology with IKKalpha and IKKbeta in the amino-terminal half, which includes the kinase domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84kDa
NCBI Official Full Name
serine/threonine-protein kinase TBK1
NCBI Official Synonym Full Names
TANK-binding kinase 1
NCBI Official Symbol
TBK1
NCBI Official Synonym Symbols
NAK; T2K
NCBI Protein Information
serine/threonine-protein kinase TBK1; NF-kB-activating kinase; NF-kappa-B-activating kinase
UniProt Protein Name
Serine/threonine-protein kinase TBK1
Protein Family
UniProt Gene Name
TBK1
UniProt Synonym Gene Names
NAK
UniProt Entry Name
TBK1_HUMAN
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Product Notes
The TBK1 tbk1 (Catalog #AAA3014722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TBK1 polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TBK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500~1:1000 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the TBK1 tbk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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