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Western Blot (WB) (All lanes : Anti-NEFH Antibody (Center) at 1:500-1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: Neuro-2a whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 112 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human, Mouse NEFH Polyclonal Antibody | anti-NEFH antibody

NEFH Antibody (Center)

Gene Names
NEFH; NFH; CMT2CC
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
NEFH; Polyclonal Antibody; NEFH Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein; KIAA0845; NFH; anti-NEFH antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This NEFH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 328-362 amino acids of human NEFH.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
1020
Applicable Applications for anti-NEFH antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Cellular Location
Cytoplasm.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-NEFH Antibody (Center) at 1:500-1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: Neuro-2a whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 112 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-NEFH Antibody (Center) at 1:500-1:2000 dilutionLane 1: SH-SY5Y whole cell lysateLane 2: Neuro-2a whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 112 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-NEFH antibody
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
References
Lees J.F.,et al.EMBO J. 7:1947-1955(1988). Zhu Y.,et al.Beijing Yi Ke Da Xue Xue Bao 31:531-531(1999). Nagase T.,et al.DNA Res. 5:355-364(1998). Ota T.,et al.Nat. Genet. 36:40-45(2004). Dunham I.,et al.Nature 402:489-495(1999).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
112479
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament heavy
NCBI Official Symbol
NEFH
NCBI Official Synonym Symbols
NFH; CMT2CC
NCBI Protein Information
neurofilament heavy polypeptide
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Gene Name
NEFH
UniProt Synonym Gene Names
KIAA0845; NFH; NF-H

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

Research Articles on NEFH

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Product Notes

The NEFH nefh (Catalog #AAA9231288) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEFH Antibody (Center) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NEFH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NEFH nefh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEFH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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