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Western Blot (WB) (Western BlotPositive WB detected in:Mouse brain tissue,Mouse stomach tissueAll lanes: TBC1D20 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 46,20,49 kDaObserved band size: 46 kDa)

Rabbit anti-Human TBC1D20 Polyclonal Antibody | anti-TBC1D20 antibody

TBC1D20 Antibody

Gene Names
TBC1D20; WARBM4; C20orf140
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G Purified
Synonyms
TBC1D20; Polyclonal Antibody; TBC1D20 Antibody; TBC1 domain family member 20; C20orf140; anti-TBC1D20 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G Purified
Sequence Length
403
Applicable Applications for anti-TBC1D20 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Recommended dilution: IHC:1:20-1:200
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human TBC1 domain family member 20 protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-85951
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse brain tissue,Mouse stomach tissueAll lanes: TBC1D20 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 46,20,49 kDaObserved band size: 46 kDa)

Western Blot (WB) (Western BlotPositive WB detected in:Mouse brain tissue,Mouse stomach tissueAll lanes: TBC1D20 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 46,20,49 kDaObserved band size: 46 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis using MBS1496665 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis using MBS1496665 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS1496665 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS1496665 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells using MBS1496665 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of MCF-7 cells using MBS1496665 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-TBC1D20 antibody
GTPase-activating protein specific for Rab1 and Rab2 small GTPase families for which it can accelerate the intrinsic GTP hydrolysis rate by more than five orders of magnitude.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,382 Da
NCBI Official Full Name
TBC1 domain family member 20
NCBI Official Synonym Full Names
TBC1 domain family member 20
NCBI Official Symbol
TBC1D20
NCBI Official Synonym Symbols
WARBM4; C20orf140
NCBI Protein Information
TBC1 domain family member 20
UniProt Protein Name
TBC1 domain family member 20
Protein Family
UniProt Gene Name
TBC1D20
UniProt Synonym Gene Names
C20orf140
UniProt Entry Name
TBC20_HUMAN

NCBI Description

This gene encodes a protein that belongs to a family of GTPase activator proteins of Rab-like small GTPases. The encoded protein and its cognate GTPase, Rab1, bind the nonstructural protein 5A (NS5A) of the hepatitis C virus (HCV) to mediate viral replication. Depletion of this protein inhibits replication of the virus and HCV infection. Mutations in this gene are associated with Warburg micro syndrome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

TBC1D20: May act as a GTPase-activating protein for Rab family protein(s). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GAPs; GAPs, Rab

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to Golgi membrane; nuclear membrane

Molecular Function: GTPase activator activity; protein binding; Rab GTPase binding

Biological Process: acrosome formation; ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; positive regulation of GTPase activity; positive regulation of viral protein levels in host cell; virus assembly

Disease: Warburg Micro Syndrome 4

Research Articles on TBC1D20

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Product Notes

The TBC1D20 tbc1d20 (Catalog #AAA1496665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TBC1D20 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TBC1D20 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Recommended dilution: IHC:1:20-1:200. Researchers should empirically determine the suitability of the TBC1D20 tbc1d20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBC1D20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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