Rabbit anti-Human TBC1D10B Polyclonal Antibody | anti-TBC1D10B antibody

TBC1D10B, CT (TBC1D10B, TBC1 domain family member 10B, Rab27A-GAP-beta) (Biotin)

Cat. Num. AAA6353844

• Gene Names: TBC1D10B; EPI64B; FP2461
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBC1D10B; Polyclonal Antibody; CT (TBC1D10B; TBC1 domain family member 10B; Rab27A-GAP-beta) (Biotin); Rab27A-GAP-beta; anti-TBC1D10B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TBC1D10B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TBC1D10B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 780-808 amino acids from the C-terminal region of human TBC1D10B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TBC1D10B Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TBC1D10B antibody detected the TBC1D10B protein (arrow).)

Related Product Information for anti-TBC1D10B antibody

Small G proteins of the RAB family (see MIM 179508) function in intracellular vesicle trafficking by switching from the GTP-bound state to the GDP-bound state with the assistance of guanine nucleotide exchange factors (GEFs; see MIM 609700) and GTPase-activating proteins (GAPs). TBC1D10B functions as a GAP for several proteins of the Rab family (Ishibashi et al., 2009 [PubMed 19077034]).

Product Categories/Family for anti-TBC1D10B antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Ishibashi, K., et al. Genes Cells 14(1):41-52(2009). Choy, K.W., et al. Physiol. Genomics 25(1):9-15(2006). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 167614488
• NCBI GeneID: 26000
• NCBI Accession #: NP_056342.3
• NCBI GenBank Nucleotide #: NM_015527.3
• UniProt Accession #: Q4KMP7; Q6IN54; Q6P530; Q71RG7; Q86VC5; Q9H8Z2; Q9NUN6; Q9UFP2; B9A6L0
• Molecular Weight: 87,199 Da
• NCBI Official Full Name: TBC1 domain family member 10B
• NCBI Official Synonym Full Names: TBC1 domain family, member 10B
• NCBI Official Symbol: TBC1D10B
• NCBI Official Synonym Symbols: EPI64B; FP2461
• NCBI Protein Information: TBC1 domain family member 10B; Rab27A-GAPbeta; rab27A-GAP-beta
• UniProt Protein Name: TBC1 domain family member 10B
• Protein Family: TBC1 domain family
• UniProt Gene Name: TBC1D10B
• UniProt Entry Name: TB10B_HUMAN

NCBI Description

Small G proteins of the RAB family (see MIM 179508) function in intracellular vesicle trafficking by switching from the GTP-bound state to the GDP-bound state with the assistance of guanine nucleotide exchange factors (GEFs; see MIM 609700) and GTPase-activating proteins (GAPs). TBC1D10B functions as a GAP for several proteins of the Rab family (Ishibashi et al., 2009 [PubMed 19077034]).[supplied by OMIM, Nov 2010]

Uniprot Description

Function: Acts as GTPase-activating protein for RAB3A, RAB22A, RAB27A, AND RAB35. Does not act on RAB2A and RAB6A. Ref.6 Ref.9

Subcellular location: Cytoplasm. Note: In melanocytes, located at the periphery of cells. Ref.9

Sequence similarities: Contains 1 Rab-GAP TBC domain.

Sequence caution: The sequence AAH50523.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH63112.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH72453.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH93814.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH93816.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH98419.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAQ15206.1 differs from that shown. Reason: Frameshift at positions 154, 379 and 401. The sequence BAA92086.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAB14454.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAH16638.1 differs from that shown. Reason: Probable cloning artifact.The sequence EAW52256.1 differs from that shown. Reason: Erroneous gene model prediction.