Rabbit anti-Human TAZ Polyclonal Antibody | anti-TAZ antibody

TAZ, NT (Protein G4.5, Tafazzin, EFE2, G4.5) (FITC)

Cat. Num. AAA6502132

• Gene Names: TAZ; EFE; BTHS; EFE2; G4.5; Taz1; CMD3A; LVNCX
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: TAZ; Polyclonal Antibody; NT (Protein G4.5; Tafazzin; EFE2; G4.5) (FITC); anti-TAZ antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAZ.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TAZ antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human TAZ (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TAZ Antibody (N-term) in MDA-MB231, NCI-H460 cell line lysates (35ug/lane). TAZ (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with TAZ Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TAZ Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TAZ antibody

Antibodies; Cardiac Markers

References

1. Gedeon, A.K., et.al., J. Med. Genet. 32(5), 383-388 (1995). 2. Bione, S., et.al., Nat. Genet. 12(4), 385-389 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4507371
• NCBI GeneID: 6901
• NCBI Accession #: NP_000107.1
• NCBI GenBank Nucleotide #: NM_000116.3
• UniProt Accession #: Q16635; Q5HY43; Q5HY44; Q5HY45; Q5HY48; Q86XQ6; Q86XQ7; Q86XQ8; Q86XQ9; Q86XR0; A3KQT2; D3DWX2
• Molecular Weight: 33,459 Da
• NCBI Official Full Name: tafazzin isoform 1
• NCBI Official Synonym Full Names: tafazzin
• NCBI Official Symbol: TAZ
• NCBI Official Synonym Symbols: EFE; BTHS; EFE2; G4.5; Taz1; CMD3A; LVNCX
• NCBI Protein Information: tafazzin; protein G4.5
• UniProt Protein Name: Tafazzin
• Protein Family: Tafazzin
• UniProt Gene Name: TAZ
• UniProt Synonym Gene Names: EFE2; G4.5
• UniProt Entry Name: TAZ_HUMAN

NCBI Description

This gene encodes a protein that is expressed at high levels in cardiac and skeletal muscle. Mutations in this gene have been associated with a number of clinical disorders including Barth syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibroelastosis, and left ventricular noncompaction (LVNC). Multiple transcript variants encoding different isoforms have been described. A long form and a short form of each of these isoforms is produced; the short form lacks a hydrophobic leader sequence and may exist as a cytoplasmic protein rather than being membrane-bound. Other alternatively spliced transcripts have been described but the full-length nature of all these transcripts is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

tafazzin: Some isoforms may be involved in cardiolipin (CL) metabolism. Defects in TAZ are the cause of Barth syndrome (BTHS). An X-linked disease characterized by dilated cardiomyopathy with endocardial fibroelastosis, a predominantly proximal skeletal myopathy, growth retardation, neutropenia, and organic aciduria, particularly excess of 3-methylglutaconic acid. Additional features include hypertrophic cardiomyopathy, isolated left ventricular non-compaction, ventricular arrhythmia, motor delay, poor appetite, fatigue and exercise intolerance, hypoglycemia, lactic acidosis, hyperammonemia, and dramatic late catch-up growth after growth delay throughout childhood. Belongs to the taffazin family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; Mitochondrial

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial membrane; integral to membrane; intrinsic to membrane; cytosol; nucleus

Molecular Function: 1-acylglycerophosphocholine O-acyltransferase activity

Biological Process: cardiac muscle development; cardiolipin biosynthetic process; mitochondrial respiratory chain complex I assembly; skeletal muscle development; cristae formation; muscle contraction; heart development; phospholipid metabolic process; glycerophospholipid biosynthetic process; hemopoiesis; cardiac muscle contraction; organelle ATP synthesis coupled electron transport

Disease: Barth Syndrome