Western Blot (WB)
(Western Blot: Sample: Recombinant TAF1, Human.)
Rabbit anti-Human TATA Box Binding Protein Associated Factor 1 (TAF1) Polyclonal Antibody | anti-TAF1 antibody
Polyclonal Antibody to TATA Box Binding Protein Associated Factor 1 (TAF1)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TD PMVTLSSILE SIINDMRDLP NTYPFHTPVN AKVVKDYYKI ITRPMDLQTL RENVRKRLYP SREEFREHLE LIVKNSATYN GPKHSLTQIS QSMLDLCDEK LKEKEDKLAR LEKAINPLLD DDDQVAFSFI LDNIVTQKMM AVPDSWPFHH PVNKKFVPDY YKVIVNPMDL ETIRKNISKH KYQSRESFLD DVNLILANSV
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant TAF1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TAF1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TAF1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TAF1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue))
NCBI and Uniprot Product Information
NCBI Description
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]
Uniprot Description
TAF1: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA- binding activity. Essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3); also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. Belongs to the TAF1 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: ATYPICAL group; DNA-binding; EC 2.3.1.48; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; TAF1 family; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: MLL1 complex; nuclear chromatin; nucleolus; nucleoplasm; nucleus; transcription factor complex; transcription factor TFIID complex
Molecular Function: acetyl-CoA binding; ATP binding; histone acetyltransferase activity; kinase activity; p53 binding; protein binding; protein heterodimerization activity; protein serine/threonine kinase activity; sequence-specific DNA binding; TATA-binding protein binding; transcription coactivator activity; transcription factor binding; transcription regulatory region DNA binding
Biological Process: cell cycle; cellular response to ATP; cellular response to DNA damage stimulus; cellular response to UV; DNA-templated transcription, initiation; histone acetylation; midbrain development; negative regulation of gene expression; negative regulation of protein autoubiquitination; negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of androgen receptor activity; positive regulation of cell proliferation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; protein autophosphorylation; protein polyubiquitination; protein stabilization; protein ubiquitination involved in ubiquitin-dependent protein catabolic process; regulation of cell cycle G1/S phase transition; regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage; RNA polymerase II transcriptional preinitiation complex assembly; transcription elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral process
Disease: Dystonia 3, Torsion, X-linked; Mental Retardation, X-linked, Syndromic 33
Research Articles on TAF1
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Product Notes
The TAF1 taf1 (Catalog #AAA2016162) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to TATA Box Binding Protein Associated Factor 1 (TAF1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TATA Box Binding Protein Associated Factor 1 (TAF1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TAF1 taf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TD PMVTLSSILE SIINDMRDLP NTYPFHTPVN AKVVKDYYKI ITRPMDLQTL RENVRKRLYP SREEFREHLE LIVKNSATYN GPKHSLTQIS QSMLDLCDEK LKEKEDKLAR LEKAINPLLD DDDQVAFSFI LDNIVTQKMM AVPDSWPFHH PVNKKFVPDY YKVIVNPMDL ETIRKNISKH KYQSRESFLD DVNLILANSV. It is sometimes possible for the material contained within the vial of "TATA Box Binding Protein Associated Factor 1 (TAF1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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