anti-Human, Mouse TAF1 Antibody | anti-TAF1 antibody

TAF1 Antibody

Cat. Num. AAA853858

• Gene Names: TAF1; OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; MRXS33; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: TAF1; TAF1 Antibody; BA2R; CCG1; Cell cycle gene 1 protein; EC 2.7.11.1; P250; T2D1; TAF2A; TAFII-250; TAFII250; TBP-associated factor 250 kDa; Transcription initiation factor TFIID 250 kDa subunit; kinase TAF1; anti-TAF1 antibody

• Reactivity: Human, Mouse
• Specificity: TAF1 antibody detects endogenous levels of total TAF1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1895

• Applicable Applications for anti-TAF1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human TAF1.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TAF1 antibody.)

Product Categories/Family for anti-TAF1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Sekiguchi T., Mol. Cell. Biol. 11:3317-3325(1991). Ruppert S., Nature 362:175-179(1993). Nolte D., Proc. Natl. Acad. Sci. U.S.A. 100:10347-10352(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 554503966
• NCBI GeneID: 6872
• NCBI Accession #: NP_001273003.1
• NCBI GenBank Nucleotide #: NM_001286074.1
• UniProt Accession #: P21675; Q59FZ3; Q6IUZ1; Q70Q86; Q70Q87; Q70T00; Q70T01; A5CVC8; A5CVC9; A5CVD0; A5CVD1; B1Q2X3
• Molecular Weight: 214,913 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 1 isoform 3
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 1
• NCBI Official Symbol: TAF1
• NCBI Official Synonym Symbols: OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; MRXS33; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250
• NCBI Protein Information: transcription initiation factor TFIID subunit 1
• UniProt Protein Name: Transcription initiation factor TFIID subunit 1
• Protein Family: Taz1-interacting factor
• UniProt Gene Name: TAF1
• UniProt Synonym Gene Names: BA2R; CCG1; CCGS; TAF2A; p250; TAF(II)250; TAFII-250; TAFII250

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]

Uniprot Description

Largest component and core scaffold of the TFIID basal transcription factor complex (PubMed:25412659, PubMed:27007846). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity (PubMed:25412659). Essential for progression of the G1 phase of the cell cycle (PubMed:11278496, PubMed:15053879, PubMed:2038334, PubMed:8450888, PubMed:8625415, PubMed:9660973, PubMed:9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed:15870300).