Western Blot (WB)
(Western Blot analysis in Hela cell line and mouse brain lysates (35ug/lane) usingMBS646168. This demonstrates MBS646168 detected the TARDBP protein (arrow).)
Rabbit anti-Human, Mouse TARDBP Polyclonal Antibody | anti-TARDBP antibody
TARDBP, NT (TARDBP, TDP43, TAR DNA-binding protein 43) (MaxLight 550)
Gene Names
TARDBP; ALS10; TDP-43
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TARDBP; Polyclonal Antibody; NT (TARDBP; TDP43; TAR DNA-binding protein 43) (MaxLight 550); TAR DNA-binding protein 43; anti-TARDBP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TARDBP at ~45kD. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-TARDBP antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa1-30 in the N-terminal region of human TARDBP, Swiss/UniProt Accession: Q13148 Cellular Localization: Nucleus. Species sequence homology: chicken
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in Hela cell line and mouse brain lysates (35ug/lane) usingMBS646168. This demonstrates MBS646168 detected the TARDBP protein (arrow).)
Western Blot (WB)
(Western Blot analysis in Hela cell line and mouse brain lysates (35ug/lane) usingMBS646168. This demonstrates MBS646168 detected the TARDBP protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue using MBS646168 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates MBS646168 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue using MBS646168 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates MBS646168 for immunohistochemistry.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained withMBS646168. Hela cells were fixed with 4% PFA (20min), permeabilized with Triton X-100 (0.1%, 10min), then incubated with MBS646168 (1:25, 1hr at 37 degree C). For secondary antibody, Alexa Fluor488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1hr at 37 degree C). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10min). TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained withMBS646168. Hela cells were fixed with 4% PFA (20min), permeabilized with Triton X-100 (0.1%, 10min), then incubated with MBS646168 (1:25, 1hr at 37 degree C). For secondary antibody, Alexa Fluor488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50min at 37 degree C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1hr at 37 degree C). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10min). TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)
Product Categories/Family for anti-TARDBP antibody
References
1. Kim, S.H., et al. J. Biol. Chem. 285(44):34097-34105(2010). 2. Geser, F., et al. Arch. Neurol. 67(10):1238-1250(2010). 3. Mackenzie, I.R., et al. Lancet Neurol 9(10):995-1007(2010). 4. Shan, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16325-16330(2010). 5. McKee, A.C., et al. J. Neuropathol. Exp. Neurol. 69(9):918-929(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
414
NCBI Official Full Name
TAR DNA-binding protein 43
NCBI Official Synonym Full Names
TAR DNA binding protein
NCBI Official Symbol
TARDBP
NCBI Official Synonym Symbols
ALS10; TDP-43
NCBI Protein Information
TAR DNA-binding protein 43; TAR DNA-binding protein-43
UniProt Protein Name
TAR DNA-binding protein 43
Protein Family
UniProt Gene Name
TARDBP
UniProt Synonym Gene Names
TDP43; TDP-43
UniProt Entry Name
TADBP_HUMAN
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Product Notes
The TARDBP tardbp (Catalog #AAA6353607) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TARDBP, NT (TARDBP, TDP43, TAR DNA-binding protein 43) (MaxLight 550) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TARDBP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TARDBP tardbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TARDBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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