Rabbit anti-Human TARBP2 Polyclonal Antibody | anti-TARBP2 antibody

TARBP2, NT (TARBP2, TRBP, RISC-loading complex subunit TARBP2, TAR RNA-binding protein 2, Trans-activation-responsive RNA-binding protein) (APC)

Cat. Num. AAA6353590

• Gene Names: TARBP2; LOQS; TRBP; TRBP1; TRBP2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TARBP2; Polyclonal Antibody; NT (TARBP2; TRBP; RISC-loading complex subunit TARBP2; TAR RNA-binding protein 2; Trans-activation-responsive RNA-binding protein) (APC); Trans-activation-responsive RNA-binding protein; anti-TARBP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TARBP2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TARBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-68 amino acids from the N-terminal region of human TARBP2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TARBP2 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the TARBP2 antibody detected the TARBP2 protein (arrow).)

Immunohistochemistry (IHC)

(TARBP2 Antibody (N-term) (AP14108a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TARBP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TARBP2 antibody

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Product Categories/Family for anti-TARBP2 antibody

Antibodies; Binding Proteins

References

Garre, P., et al. Nat. Genet. 42(10):817-818(2010). Wilker, E.H., et al. Environ. Health Perspect. 118(7):943-948(2010). Boni, V., et al. Pharmacogenomics J. (2010). In press: Wang, H.W., et al. Nat. Struct. Mol. Biol. 16(11):1148-1153(2009). Lau, P.W., et al. Structure 17(10):1326-1332(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 85068512
• NCBI GeneID: 6895
• NCBI Accession #: NP_599151.2; NP_599150.1
• NCBI GenBank Nucleotide #: NM_134324.2
• UniProt Accession #: Q15633; Q12878; Q8WY32; Q8WY33; Q9BRY2
• Molecular Weight: 36,916 Da
• NCBI Official Full Name: RISC-loading complex subunit TARBP2 isoform b
• NCBI Official Synonym Full Names: TAR (HIV-1) RNA binding protein 2
• NCBI Official Symbol: TARBP2
• NCBI Official Synonym Symbols: LOQS; TRBP; TRBP1; TRBP2
• NCBI Protein Information: RISC-loading complex subunit TARBP2
• UniProt Protein Name: RISC-loading complex subunit TARBP2
• Protein Family: RISC-loading complex
• UniProt Gene Name: TARBP2
• UniProt Entry Name: TRBP2_HUMAN

NCBI Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRBP: the HIV-1 TAR RNA binding protein (TRBP) is a double strand RNA binding protein (dsRBP) that is required for the recruitment of Ago2 to the small interfering RNA (siRNA) bound by Dicer. TRBP increases HIV-1 expression and replication by inhibiting the interferon-induced protein kinase PKR and by increasing translation of viral mRNA. Dicer, Ago2, and TRBP are the main components of the RNA-induced silencing complex (RISC).

Protein type: Translation; Motility/polarity/chemotaxis; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; siRNA binding; protein homodimerization activity; miRNA binding; double-stranded RNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of translation; positive regulation of viral genome replication; regulation of transcription, DNA-dependent; pre-microRNA processing; regulation of viral transcription; RNA interference, targeting of mRNA for destruction; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of protein kinase activity; gene expression; negative regulation of defense response to virus by host; RNA interference, production of siRNA