Rabbit Histone H3.1 Polyclonal Antibody | anti-HIST1H3A antibody

Histone H3.1 (HIST1H3J, Histone H3R26me2a, H3/j, H3FJ, Histone H3/a, Histone H3/b,Histone H3/c, Histone H3/d, Histone H3/f,Histone H3/h, Histone H3/I,Histone H3/j, _+E_Histone H3/k, Histone H3/l) (FITC)

Cat. Num. AAA6420724

• Gene Names: HIST1H3A; H3/A; H3FA
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunoprecipitation
• Purity: Purified by affinity chromatography.
• Synonyms: Histone H3.1; Polyclonal Antibody; Histone H3.1 (HIST1H3J; Histone H3R26me2a; H3/j; H3FJ; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; Histone H3/j; _+E_Histone H3/k; Histone H3/l) (FITC); anti-HIST1H3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Histone H3R26me2a. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-HIST1H3A antibody: Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IP: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Synthetic methylated peptide corresponding to residues surrounding Arg26 of _+E_human histone H3.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3R26me2a antibody.)

Dot Blot (DB)

(Dot-blot analysis of all sorts of methylation peptides using H3R26 me2a antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of 293T cell using H3R26 me2a antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-HIST1H3A antibody

Antibodies; Histones

NCBI and Uniprot Product Information

• NCBI GI #: 4504281
• NCBI GeneID: 8350
• NCBI Accession #: NP_003520.1
• NCBI GenBank Nucleotide #: NM_003529.2
• UniProt Accession #: P68431; P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
• Molecular Weight: 15,404 Da
• NCBI Official Full Name: histone H3.1
• NCBI Official Synonym Full Names: histone cluster 1, H3a
• NCBI Official Symbol: HIST1H3A
• NCBI Official Synonym Symbols: H3/A; H3FA
• NCBI Protein Information: histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A
• UniProt Protein Name: Histone H3.1
• Protein Family: Histone
• UniProt Gene Name: HIST1H3A
• UniProt Synonym Gene Names: H3FA
• UniProt Entry Name: H31_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Uniprot Description

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; DNA methylation on cytosine