Rabbit Tapasin Polyclonal Antibody | anti-TAPBP antibody

Tapasin (TPN, TPSN, NGS17, NGS-17, TAP-associated Protein, TAPA, TAP-binding Protein, TAPBP)

Cat. Num. AAA611508

• Gene Names: TAPBP; TPN; TAPA; TPSN; NGS17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography
• Synonyms: Tapasin; Polyclonal Antibody; Tapasin (TPN; TPSN; NGS17; NGS-17; TAP-associated Protein; TAPA; TAP-binding Protein; TAPBP); Anti -Tapasin (TPN; anti-TAPBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects an ~48kD protein, corresponding to the apparent molecular mass of tapasin on SDS-PAGE immunoblots, in samples from human origin.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography
• Form/Format: Supplied as a liquid. in PBS, pH 7.2, 0.09% azide, 50% glycerol

• Applicable Applications for anti-TAPBP antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Dilution: Western Blot (ECL): 1:1000; Immunoprecipitation: 1:100; Optimal dilutions to be determined by researcher.
• Immunogen: Human tapasin synthetic peptide (residues 430-448), with a cysteine (C) residue added.
• Positive Control: HeLa Cell Lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis using MBS611508 Lane 1: MW marker, Lane 2: A431 Lysate)

Related Product Information for anti-TAPBP antibody

Tapasin is a 48kD glycoprotein which is a member of the immunoglobulin (Ig) superfamily and it is required for efficient peptide binding to Transporter associated with Antigen Processing (TAP). TAP binds peptides in its cytolic part and subsequently translocates the peptides into the lumen of the endoplasmic reticulum (ER) where assembly of MHC class I and peptide takes place. Assembly of MHC class I-beta2-microglobulin (beta2-m) dimers in the ER involves 2 chaperones, calnexin which interacts with free class I heavy (H) chains and calreticulin which binds human class I-beta2 dimers prior to peptide loading. Calreticulin remains associated with at least a subset of class I molecules when they in turn bind to TAP (1). Polymorphic differences in MHC class I H chains can result in quantitative as well as qualitative differences in how they interact with peptide, beta2-m, calnexin, calreticulin, Erp57, TAP and Tapasin, a subunit of the TAP complex which binds to both TAP1 and MHC class I (2,3). Data obtained with Tapasin deletion mutants revealed that binding to TAP is mediated by the C-terminal region and that the N-terminal region is required to stabilize the MHC class I loading complex (4). The Tapasin gene is centromeric of HLA-DP locus between the HSET and HKE1.5 genes and within 500kbp of the transporters associated with antigen processing, TAP1 and TAP2 genes. The localization of these genes within such a short distance of each other on the chromosome implies some regulatory or functional significance (5).

Product Categories/Family for anti-TAPBP antibody

Antibodies; Abs to Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 11225591
• NCBI GeneID: 6892
• UniProt Accession #: O15533; O15210; O15272; Q5STJ8; Q5STK6; Q5STQ5; Q5STQ6; Q66K65; A2AB91; A2ABC0; B0V003; B0V0A6; Q5HYV0; Q6P1N7
• Molecular Weight: 47,626 Da
• NCBI Official Full Name: tapasin
• NCBI Official Synonym Full Names: TAP binding protein (tapasin)
• NCBI Official Symbol: TAPBP
• NCBI Official Synonym Symbols: TPN; TAPA; TPSN; NGS17
• NCBI Protein Information: tapasin; NGS-17; OTTHUMP00000029102; OTTHUMP00000178697; OTTHUMP00000178698; OTTHUMP00000178699; TAP-binding protein; TAP-associated protein
• UniProt Protein Name: Tapasin
• Protein Family: Tapasin
• UniProt Gene Name: TAPBP
• UniProt Synonym Gene Names: NGS17; TAPA
• UniProt Entry Name: TPSN_HUMAN

NCBI Description

This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

TAPBP: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; TAP complex; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; peptide antigen binding; TAP1 binding; MHC class I protein binding; unfolded protein binding; TAP2 binding; peptide antigen-transporting ATPase activity

Biological Process: peptide transport; antigen processing and presentation of peptide antigen via MHC class I; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; peptide antigen stabilization; immune response; protein complex assembly; amide transport; antigen processing and presentation of endogenous peptide antigen via MHC class I

Disease: Bare Lymphocyte Syndrome, Type I