Rabbit anti-Human TAP1 Polyclonal Antibody | anti-TAP1 antibody

TAP1, CT (Antigen Peptide Transporter 1, APT1, Peptide Transporter TAP1, ATP-Binding Cassette Sub-family B Member 2, Peptide Supply Factor 1, Peptide Transporter PSF1, PSF-1, Peptide Transporter Involved In Antigen Processing 1, Really Interesting New Gen

Cat. Num. AAA6013135

• Gene Names: TAP1; APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: TAP1; Polyclonal Antibody; CT (Antigen Peptide Transporter 1; APT1; Peptide Transporter TAP1; ATP-Binding Cassette Sub-family B Member 2; Peptide Supply Factor 1; Peptide Transporter PSF1; PSF-1; Peptide Transporter Involved In Antigen Processing 1; Really Interesting New Gen; Anti -TAP1; anti-TAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAP1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-TAP1 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human TAP1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(TAP1 Antibody (I795) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the TAP1 antibody detected the TAP1 protein (arrow).)

Related Product Information for anti-TAP1 antibody

TAP is an integral transmembrane protein involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. TAP is inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. It is inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP and prevents the conformational rearrangement of TAP induced by peptide binding. TAP is also inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP is down-regulated by human Epstein-barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. TAP1 and TAP2 form a heterodimer of TAP1 and TAP2, and the peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2. TAP, inducible by interferon gamma, belongs to the ABC transporter family, MDR subfamily.

Product Categories/Family for anti-TAP1 antibody

Antibodies; Abs to Multi Drug Resistance (MDR)

NCBI and Uniprot Product Information

• NCBI GI #: 45181465
• NCBI GeneID: 6890
• UniProt Accession #: Q03518; Q16149; Q96CP4
• Molecular Weight: 87,218 Da
• NCBI Official Full Name: TAP1
• NCBI Official Synonym Full Names: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• NCBI Official Symbol: TAP1
• NCBI Official Synonym Symbols: APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
• NCBI Protein Information: antigen peptide transporter 1; ABC transporter, MHC 1; peptide supply factor 1; peptide transporter PSF1; peptide transporter TAP1; really interesting new gene 4 protein; ATP-binding cassette sub-family B member 2; transporter associated with antigen processing; peptide transporter involved in antigen processing 1; ATP-binding cassette, sub-family B (MDR/TAP), member 2; transporter, ATP-binding cassette, major histocompatibility complex, 1
• UniProt Protein Name: Antigen peptide transporter 1
• Protein Family: Protein
• UniProt Gene Name: TAP1
• UniProt Synonym Gene Names: ABCB2; PSF1; RING4; Y3; APT1; PSF-1
• UniProt Entry Name: TAP1_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. [provided by RefSeq, Jul 2008]

Uniprot Description

TAP1: a 'transporter associated with antigen processing' (TAP) protein. Member of the ATP binding cassette (ABC) family of transmembrane transporters. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. Defects in TAP1 are a cause of bare lymphocyte syndrome.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: endoplasmic reticulum membrane; TAP complex; mitochondrion; membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; protein homodimerization activity; peptide transporter activity; peptide antigen binding; TAP1 binding; ATPase activity, coupled to transmembrane movement of substances; MHC class I protein binding; TAP2 binding; ADP binding; ATP binding; peptide-transporting ATPase activity

Biological Process: peptide transport; intracellular transport of viral proteins in host cell; antigen processing and presentation of peptide antigen via MHC class I; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; defense response; transmembrane transport; antigen processing and presentation of endogenous peptide antigen via MHC class I; cytosol to ER transport

Disease: Bare Lymphocyte Syndrome, Type I