Testing Data
(Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Rabbit anti-Human TAP Polyclonal Antibody | anti-TAP antibody
TAP antibody
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Testing Data
(Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Testing Data
(Sample (30 ug of whole cell lysate) A: Hela B: Molt-4 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using TAP antibody at 1: 100 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using TAP antibody at 1: 100 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP antibody at 1: 200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP antibody at 1: 200 dilution.)
NCBI and Uniprot Product Information
NCBI Description
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
NXF1: Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5. Belongs to the NXF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 11q12-q13
Cellular Component: cytosol; nuclear speck; nucleoplasm; nucleus
Molecular Function: nucleotide binding; protein binding; single-stranded RNA binding
Biological Process: gene expression; mRNA export from nucleus; poly(A)+ mRNA export from nucleus; viral reproduction
Research Articles on TAP
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Product Notes
The TAP nxf1 (Catalog #AAA9403013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TAP antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:250 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the TAP nxf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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