Sheep anti-Human Tamm-Horsfall Glycoprotein Polyclonal Antibody
Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD)
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
Tamm-Horsfall Glycoprotein; Polyclonal Antibody; Tamm-Horsfall Glycoprotein (Uromucoid; THgp; THP; Uromodulin; UMOD); Anti -Tamm-Horsfall Glycoprotein (Uromucoid; anti-Tamm-Horsfall Glycoprotein antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Tamm-Horsfall Glycoprotein. No significant crossreactivity with urinary or other related human serum proteins. No known crossreactivity with other serum proteins in humans or other species.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in Glycine Buffered Saline, pH 7.4, 0.1% sodium azide, 0.1% EACA, 0.01% Benzamidine, 1mM EDTA, before the addition of glycerol to 40%.
Applicable Applications for anti-Tamm-Horsfall Glycoprotein antibody
ELISA (EL/EIA), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry and ELISA.
Dilution: Immunohistochemistry (Frozen sections only):1:25- 1:100.
ELISA: 1:5000- 1:20,000.
Dilution: Immunohistochemistry (Frozen sections only):1:25- 1:100.
ELISA: 1:5000- 1:20,000.
Immunogen
Native purified human Tamm-Horsfall glycoprotein (uromucoid) from urine.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Tamm-Horsfall Glycoprotein antibody
Tamm-Horsfall glycoprotein (THP), which is synthesized by renal tubular cells, is the most abundant protein in normal human urine. Although its physiological function remains unclear, it has been proposed that THP may act as a defence factor against urinary tract infections by inhibiting the binding of S- and P-fimbriated Escherichia coli to renal epithelial cells. Because THP-related proteins are also found in the superficial layers of the oral mucosa, THP may interfere with the cytoadherence of pathogenic bacteria that colonize mucosal surfaces other than those of the urogenital tract. It has been shown that THP binds to virulent Bordetella pertussis and reduces its adherence to both renal and pulmonary epithelial cells. This cytoadherence inhibitory effect was not observed with a B. pertussis mutant lacking the pertussis toxin (PTX) operon, and was dependent on the direct interaction of THP with the S2 subunit within the PTX B oligomer. It has also been shown that the glycosylation moiety of THP is crucial for its binding to PTX. The THP-PTX interaction was exploited to develop an affinity chromatography method that allows a one-step purification of active PTX. These observations suggest that besides its anti-adherence activity, THP may also trap toxins produced by pathogenic bacteria that colonize mucosal surfaces.
Product Categories/Family for anti-Tamm-Horsfall Glycoprotein antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Tamm-Horsfall protein - rat
Similar Products
Product Notes
The Tamm-Horsfall Glycoprotein (Catalog #AAA615852) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Tamm-Horsfall Glycoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry and ELISA. Dilution: Immunohistochemistry (Frozen sections only):1:25- 1:100. ELISA: 1:5000- 1:20,000. Researchers should empirically determine the suitability of the Tamm-Horsfall Glycoprotein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tamm-Horsfall Glycoprotein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
