Western Blot (WB)
(Western Blot (WB) analysis of TAGLN polyclonal antibody)
Rabbit TAGLN Polyclonal Antibody | anti-TAGLN antibody
TAGLN Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
TAGLN; Polyclonal Antibody; TAGLN Polyclonal Antibody; Transgelin; 22 kDa actin-binding protein; Protein WS3-10; Smooth muscle protein 22-alpha; SM22-alpha; SM22; WS3-10; anti-TAGLN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TAGLN polyclonal antibody detects endogenous levels of TAGLN protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
201
Applicable Applications for anti-TAGLN antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human TAGLN.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of TAGLN polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of TAGLN polyclonal antibody)
Related Product Information for anti-TAGLN antibody
Transgelin, also designated SM22a, is expressed abundantly in smooth muscle cells. The human transgelin gene (designated TAGLN), which is located on chromosome 11q23.3, encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1: 6 Actin monomers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 23kDa
NCBI Official Full Name
transgelin
UniProt Protein Name
Transgelin
Protein Family
UniProt Gene Name
TAGLN
UniProt Synonym Gene Names
SM22; WS3-10; SM22-alpha
UniProt Entry Name
TAGL_HUMAN
Uniprot Description
TAGLN: Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. Belongs to the calponin family.
Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Actin-binding
Chromosomal Location of Human Ortholog: 11q23.2
Cellular Component: cytoplasm
Molecular Function: actin filament binding; protein binding
Biological Process: muscle development; epithelial cell differentiation
Similar Products
Product Notes
The TAGLN tagln (Catalog #AAA3011720) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TAGLN Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TAGLN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the TAGLN tagln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAGLN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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