Rabbit anti-Human TAGAP Polyclonal Antibody | anti-TAGAP antibody

TAGAP, ID (TAGAP, TAGAP1, T-cell activation Rho GTPase-activating protein, T-cell activation GTPase-activating protein) (APC)

Cat. Num. AAA6353469

• Gene Names: TAGAP; FKSG15; IDDM21; TAGAP1; ARHGAP47
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAGAP; Polyclonal Antibody; ID (TAGAP; TAGAP1; T-cell activation Rho GTPase-activating protein; T-cell activation GTPase-activating protein) (APC); T-cell activation GTPase-activating protein; anti-TAGAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 553

• Applicable Applications for anti-TAGAP antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TAGAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-129 amino acids from the Central region of human TAGAP.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TAGAP Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TAGAP antibody detected the TAGAP protein (arrow).)

Immunohistochemistry (IHC)

(TAGAP antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAGAP antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TAGAP Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TAGAP antibody

This gene encodes a protein that may function as a Rho GTPase-activating protein. Transcript variants encoding distinct isoforms have been identified.

Product Categories/Family for anti-TAGAP antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Hinks, A., et al. Ann. Rheum. Dis. 69(12):2169-2172(2010). Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). Amundsen, S.S., et al. Genes Immun. 11(1):79-86(2010). Raychaudhuri, S., et al. Nat. Genet. 41(12):1313-1318(2009). Trynka, G., et al. Gut 58(8):1078-1083(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 23199971
• NCBI GeneID: 117289
• NCBI Accession #: NP_687034.1; NP_473455.2
• NCBI GenBank Nucleotide #: NP_687034.1
• UniProt Accession #: Q8N103
• NCBI Official Full Name: T-cell activation Rho GTPase-activating protein isoform a
• NCBI Official Synonym Full Names: T cell activation RhoGTPase activating protein
• NCBI Official Symbol: TAGAP
• NCBI Official Synonym Symbols: FKSG15; IDDM21; TAGAP1; ARHGAP47
• NCBI Protein Information: T-cell activation Rho GTPase-activating protein
• UniProt Protein Name: T-cell activation Rho GTPase-activating protein
• Protein Family: T-cell activation Rho GTPase-activating protein
• UniProt Gene Name: TAGAP
• UniProt Synonym Gene Names: TAGAP1
• UniProt Entry Name: TAGAP_HUMAN

NCBI Description

This gene encodes a member of the Rho GTPase-activator protein superfamily. The encoded protein may function as a Rho GTPase-activating protein. Alterations in this gene may be associated with several diseases, including rheumatoid arthritis, celiac disease, and multiple sclerosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

TAGAP: an apparent GTPase-activating protein (GAP) for Rho monomeric G proteins. May play important roles during T-cell activation. Mutations have been associated with various autoimmune conditions including rheumatoid arthritis, Crohn¿s disease, and celiac disease. Four isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: cytosol

Molecular Function: guanyl-nucleotide exchange factor activity

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity