Sheep TAFI - Thrombin Activatable Fibrinolysis Inhibitor (also called procarboxypeptidase B or U), Polyclonal Antibody | anti-Cpb2 antibody

Sheep anti-human TAFI (Thrombin Activatable Fibrinolysis Inhibitor), Peroxidase Conjugated IgG

Cat. Num. AAA512148

• Gene Names: Cpb2; CPR; Cpu; TAFI; AI255929; MGC107573; 1110032P04Rik; 4930405E17Rik
• Purity: Peroxidase conjugated IgG.; Vial containing ml of whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.2 mg.
• Synonyms: TAFI - Thrombin Activatable Fibrinolysis Inhibitor (also called procarboxypeptidase B or U); ; Polyclonal Antibody; Sheep anti-human TAFI (Thrombin Activatable Fibrinolysis Inhibitor); Peroxidase Conjugated IgG; anti-Cpb2 antibody

• Host: Sheep
• Clonality: Polyclonal
• Specificity: Prior to conjugation, this antibody was specific for TAFI as demonstrated by immunoelectrophoresis and ELISA.
• Purity/Purification: Peroxidase conjugated IgG.; Vial containing ml of whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.2 mg.
• Form/Format: IgG-HRP conjugate as a clear, slightly red-brown liquid.
• Concentration: IgG-HRP concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 14. (varies by lot)

• Application Notes: Suitable as a source of peroxidase-labeled antibodies to TAFI.
• Immunogen: Human TAFI purified from plasma.
• Buffer: A buffered stabilizer solution containing 50% (v/v) glycerol.
• Preparation and Storage: Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use. Avoid exposure to sodium azide as this is an inhibitor of peroxidase activity.

Related Product Information for anti-Cpb2 antibody

TAFI (Thrombin Activatable Fibrinolysis Inhibitor), also referred to as plasma procarboxypeptidase-B, procarboxypeptidase-U and R, circulates in plasma as a zymogen with a mass of 58,000 daltons (1-6). Proteolytic activation of TAFI yields an N-terminally derived activation peptide and the C-terminal portion corresponding to the metalloprotease, activated TAFI (TAFIa). TAFIa exhibits exopeptidase activity with carboxypeptidase B-like substrate specificity capable of catalyzing the hydrolysis of Cterminal lysine and arginine residues. Cleavage of these residues on fibrin by TAFIa attenuates clot lysis by inhibiting the formation of the ternary activation complex comprising fibrin cofactor, tPA and plasminogen, thereby inhibiting plasmin generation. Although TAFI can be activated by various proteases including thrombin and plasmin, the physiological activator is proposed to be the complex thrombinthrombomodulin since the rate of activation is stimulated 1250-fold compared to thrombin alone (4). However, the rate of TAFI activation is highly dependent upon its plasma concentration. Since TAFIa apparently plays a key role in connecting coagulation and fibrinolysis and significantly increases clot stability, determination of plasma concentration of TAFI is likely crucial to assess its subsequent potential antifibrinolytic effects1-6.

References

1. Eaton, D.L., Malloy, B.E., Tsai, S.P., Henzel, W., Drayna, D., Isolation, Molecular Cloning and Partial Characterization of a Novel Carboxypeptidase B from Human Plasma. J.Biol.Chem. 266:21833-21838, 1991.
2. Hendriks, D., Scharpe, S., van Sande, M., Lommaert, M.P., Characterization of a carboxypeptidase in human serum distinct from carboxypeptidase N. J.Clin.Chem.Clin.BioChem. 27:277-285, 1989.
3. Campbell, W., Okada, J. An Arginine specific carboxypeptidase generated in blood during coagulation of inflammation which is unrelated to carboxypeptidase N or its subunits. Biochem.Biophys.Res.Commun. 162:933-939, 1989.
4. Bajzar, L., Morser, J., Nesheim, M.E. TAFI, or procarboxypeptidase B, couples the coagulation and fibrinolytic cascades through the thrombinthrombomodulin complex. J.Biol.Chem. 271:16603-16608, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 7416967
• NCBI GeneID: 56373
• UniProt Accession #: Q9JHH6; Q5EBI3; Q9QZF0
• Molecular Weight: 48,871 Da
• NCBI Official Full Name: thrombin-activatable fibrinolysis inhibitor
• NCBI Official Synonym Full Names: carboxypeptidase B2 (plasma)
• NCBI Official Symbol: Cpb2
• NCBI Official Synonym Symbols: CPR; Cpu; TAFI; AI255929; MGC107573; 1110032P04Rik; 4930405E17Rik
• NCBI Protein Information: carboxypeptidase B2; carboxypeptidase R; carboxypeptidase U; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
• UniProt Protein Name: Carboxypeptidase B2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: Cpb2
• UniProt Synonym Gene Names: Tafi
• UniProt Entry Name: CBPB2_MOUSE

Uniprot Description

Function: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

Catalytic activity: Release of C-terminal Arg and Lys from a polypeptide.

Cofactor: Binds 1 zinc ion per subunit

Enzyme regulation: TAFI/CPB2 is unique among carboxypeptidases in that it spontaneously inactivates with a short half-life, a property that is crucial for its role in controlling blood clot lysis. The zymogen is stabilized by interactions with the activation peptide. Release of the activation peptide increases a dynamic flap mobility and in time this leads to conformational changes that disrupt the catalytic site and expose a cryptic thrombin-cleavage site present at Arg-323

Subcellular location: Secreted Ref.2.

Tissue specificity: Plasma; synthesized in the liver. Ref.2

Sequence similarities: Belongs to the peptidase M14 family.