Mouse ACC Carboxylase 1 Monoclonal Antibody | anti-ACC antibody

Mouse anti-ACC Carboxylase 1 Monoclonal Antibody

Cat. Num. AAA460475

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: The mouse IgG is purified by IgG1 affinity purification
• Synonyms: ACC Carboxylase 1; Monoclonal Antibody; Mouse anti-ACC Carboxylase 1 Monoclonal Antibody; Acetyl-CoA Carboxylase; ACC; anti-ACC antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: A3H
• Specificity: This antibody recognizes high molecular weight of ACC 1 protein. It reacts with human, rat and mouse. The other species are not tested.
• Purity/Purification: The mouse IgG is purified by IgG1 affinity purification
• Form/Format: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer

• Applicable Applications for anti-ACC antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western Blot: 0.5-2 ug/ml; ELISA: 0.1-1 ug/ml; Immunoprecipitation: 2-5 ug/ml; Immunohistochemistry: 2-5 ug/ml; ; Optimal dilutions should be determined by researchers for the specific applications.
• Antigen Preparatation: A synthetic peptide derived from C-terminus of Acetyl-CoA Carbvoxylase 1 protein.
• Positive Control: K562
• Cellular Location: Cytoplasmic
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C to 8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot

( The multiple cell lysate derived from the cell lines listed above were stained with Mouse Anti-ACC antibody (Cat#MBS460475) at 1:1000. The immunoreactive band around ~280 kDa was observed.)

Related Product Information for anti-ACC antibody

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.