Rabbit anti-Human TAF11 Polyclonal Antibody | anti-TAF11 antibody

TAF11, NT (TAF11, TAF2I, Transcription initiation factor TFIID subunit 11, TFIID subunit p30-beta, Transcription initiation factor TFIID 28kD subunit) (FITC)

Cat. Num. AAA6353339

• Gene Names: TAF11; TAF2I; PRO2134; TAFII28; MGC:15243
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF11; Polyclonal Antibody; NT (TAF11; TAF2I; Transcription initiation factor TFIID subunit 11; TFIID subunit p30-beta; Transcription initiation factor TFIID 28kD subunit) (FITC); Transcription initiation factor TFIID 28kD subunit; anti-TAF11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TAF11 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TAF11.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TAF11 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the TAF11 antibody detected the TAF11 protein (arrow).)

Related Product Information for anti-TAF11 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure.

Product Categories/Family for anti-TAF11 antibody

Antibodies; Transcription Factors

References

Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Guermah, M., et al. Mol. Cell 12(4):991-1001(2003). Mengus, G., et al. J. Biol. Chem. 275(14):10064-10071(2000). Birck, C., et al. Cell 94(2):239-249(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 5032151
• NCBI GeneID: 6882
• NCBI Accession #: NP_005634.1
• NCBI GenBank Nucleotide #: NM_005643.3
• UniProt Accession #: Q15544; Q9UHS0; B2R8R3; B4DY18
• Molecular Weight: 17,142 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 11 isoform 1
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 11
• NCBI Official Symbol: TAF11
• NCBI Official Synonym Symbols: TAF2I; PRO2134; TAFII28; MGC:15243
• NCBI Protein Information: transcription initiation factor TFIID subunit 11
• UniProt Protein Name: Transcription initiation factor TFIID subunit 11
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF11
• UniProt Synonym Gene Names: TAF2I
• UniProt Entry Name: TAF11_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]