Rabbit anti-Human TADA3L Polyclonal Antibody | anti-TADA3L antibody

TADA3L, CT (TADA3, ADA3, TADA3L, Transcriptional adapter 3, ADA3 homolog, STAF54, Transcriptional adapter 3-like) (FITC)

Cat. Num. AAA6353306

• Gene Names: TADA3; ADA3; NGG1; hADA3; STAF54; TADA3L
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TADA3L; Polyclonal Antibody; CT (TADA3; ADA3; Transcriptional adapter 3; ADA3 homolog; STAF54; Transcriptional adapter 3-like) (FITC); TADA3; Transcriptional adapter 3-like; anti-TADA3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TADA3L.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TADA3L antibody: Western Blot (WB), FLISA, Immunofluorescence (IF)
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 386-414 in the C-terminal region of human TADA3L, UniProt Accession #O75528. Species sequence homology: xenopus, bovine, zebrafish, mouse, rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis in Hela cell line lysates (35ug/lane) usingMBS6013538. This demonstrates the TADA3L antibody detected the TADA3L protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of U251 cell stained withMBS6013538. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBS6013538 (1:25, 1h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.)

Related Product Information for anti-TADA3L antibody

Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least four alternatively spliced variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq].

Product Categories/Family for anti-TADA3L antibody

Antibodies; Transcription Factors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Li, C.W., et al. Nucleic Acids Res. 38(16):5291-5303(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Hu, Y., et al. Cancer Invest. 27(3):298-306(2009). Wang, Y.L., et al. J. Biol. Chem. 283(49):33808-33815(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 507417951
• NCBI GeneID: 10474
• NCBI Accession #: NP_006345.1; NP_597814.1
• NCBI GenBank Nucleotide #: NM_006354
• UniProt Accession #: O75528; Q6FI83; Q9UFS2
• Molecular Weight: 41,393 Da
• NCBI Official Full Name: Homo sapiens transcriptional adaptor 3 (TADA3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transcriptional adaptor 3
• NCBI Official Symbol: TADA3
• NCBI Official Synonym Symbols: ADA3; NGG1; hADA3; STAF54; TADA3L
• NCBI Protein Information: transcriptional adapter 3; ADA3 homolog; ADA3-like protein
• UniProt Protein Name: Transcriptional adapter 3
• UniProt Gene Name: TADA3
• UniProt Synonym Gene Names: ADA3; TADA3L; hADA3; ADA3-like protein
• UniProt Entry Name: TADA3_HUMAN

NCBI Description

DNA-binding transcriptional activator proteins increase the rate of transcription by interacting with the transcriptional machinery bound to the basal promoter in conjunction with adaptor proteins, possibly by acetylation and destabilization of nucleosomes. The protein encoded by this gene is a transcriptional activator adaptor and a component of the histone acetyl transferase (HAT) coactivator complex which plays a crucial role in chromatin modulation and cell cycle progression. Along with the other components of the complex, this protein links transcriptional activators bound to specific promoters, to histone acetylation and the transcriptional machinery. The protein is also involved in the stabilization and activation of the p53 tumor suppressor protein that plays a role in the cellular response to DNA damage. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

TADA3L: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Also known as a coactivator for p53/TP53-dependent transcriptional activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Belongs to the NGG1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: nucleoplasm; intracellular; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; protein domain specific binding; protein binding; histone acetyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; transcription coactivator activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; mitosis; regulation of histone deacetylation; regulation of protein amino acid phosphorylation; establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of protein stability