Immunofluorescence (IF)
(MBS9602250 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human TAB182 Polyclonal Antibody | anti-TAB182 antibody
TAB182 Antibody
Gene Names
TNKS1BP1; TAB182
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TAB182; Polyclonal Antibody; TAB182 Antibody; 182 kDa tankyrase 1 binding protein; 182 kDa tankyrase-1-binding protein; FLJ45975; KIAA1741; Tankyrase 1 binding protein 1 182 kDa; Tankyrase 1 binding protein 1; Tankyrase 1 binding protein of 182 kDa; TB182_HUMAN; Tnks1bp1; anti-TAB182 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TAB182 antibody detects endogenous levels of total TAB182
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1729
Applicable Applications for anti-TAB182 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human TAB182
Subcellular Location
Nucleus. Cytoplasm > Cytoskeleton. Chromosome. Colocalizes with chromosomes during mitosis, and in the cytoplasm with cortical actin.
Tissue Specificity
Detected in testis, ovary, lung, skeletal muscle, heart, prostate and pancreas, and at very low levels in brain and peripheral blood leukocytes.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog
Similarity
Pig (91%), Bovine (82%), Horse (91%), Rabbit (100%), Dog (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9602250 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602250 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of TAB182 using 293 whole cell lysates)
Western Blot (WB)
(Western blot analysis of TAB182 using 293 whole cell lysates)
Related Product Information for anti-TAB182 antibody
Subunit Structure: Binds to the ANK repeat domain of TNKS1 and TNKS2.
Post-translational Modifications: ADP-ribosylated by TNKS1 (in vitro).
Post-translational Modifications: ADP-ribosylated by TNKS1 (in vitro).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 150 kDa
Predicted: 182 kDa
Predicted: 182 kDa
NCBI Official Full Name
182 kDa tankyrase-1-binding protein
NCBI Official Synonym Full Names
tankyrase 1 binding protein 1
NCBI Official Symbol
TNKS1BP1
NCBI Official Synonym Symbols
TAB182
NCBI Protein Information
182 kDa tankyrase-1-binding protein
UniProt Protein Name
182 kDa tankyrase-1-binding protein
UniProt Gene Name
TNKS1BP1
UniProt Synonym Gene Names
KIAA1741; TAB182
Research Articles on TAB182
Similar Products
Product Notes
The TAB182 tnks1bp1 (Catalog #AAA9602250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TAB182 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TAB182 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TAB182 tnks1bp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAB182, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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