Rabbit T2R49 Polyclonal Antibody | anti-T2R49 antibody

T2R49 (V129) Polyclonal Antibody

Cat. Num. AAA3003242

• Gene Names: TAS2R20; T2R20; T2R49; T2R56; TAS2R49
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: T2R49; Polyclonal Antibody; T2R49 (V129) Polyclonal Antibody; Taste receptor type 2 member 20; Taste receptor type 2 member 49; Taste receptor type 2 member 56; T2R56; TAS2R20; TAS2R49; anti-T2R49 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: T2R49 (V129) polyclonal antibody detects endogenous levels of T2R49 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 309

• Applicable Applications for anti-T2R49 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human T2R49.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of T2R49 (V129) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)

Related Product Information for anti-T2R49 antibody

The sense of taste provides animals with valuable information about the quality and nutritional value of food. A family of G protein-coupled receptors are involved in taste perception and include T1R, which is involved in sweet and umami taste perception and T2R, which is involved in bitter taste perception. Both types of taste receptors couple to various G proteins to initiate signal transduction cascades. T2R49 plays a role in sensing the chemical composition of the gastrointestinal content. T2R49 is expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells.

NCBI and Uniprot Product Information

• NCBI GI #: 154937355
• NCBI GeneID: 259295
• NCBI Accession #: NP_795370.2
• NCBI GenBank Nucleotide #: NP_795370.2
• UniProt Accession #: P59543
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: taste receptor type 2 member 20
• NCBI Official Synonym Full Names: taste 2 receptor member 20
• NCBI Official Symbol: TAS2R20
• NCBI Official Synonym Symbols: T2R20; T2R49; T2R56; TAS2R49
• NCBI Protein Information: taste receptor type 2 member 20
• UniProt Protein Name: Taste receptor type 2 member 20
• UniProt Gene Name: TAS2R20
• UniProt Synonym Gene Names: TAS2R49; T2R49; T2R56
• UniProt Entry Name: T2R20_HUMAN

NCBI Description

This gene encodes a member of the taste receptor two family of class C G-protein coupled receptors. Receptors of this family have a short extracellular N-terminus, seven transmembrane helices, three extracellular loops and three intracellular loops, and an intracellular C-terminus. Members of this family are expressed in a subset of taste receptor cells, where they function in bitter taste reception, as well as in non-gustatory cells including those of the brain, reproductive organs, respiratory system, and gastrointestinal system. This gene maps to the taste receptor gene cluster on chromosome 12p13. [provided by RefSeq, Jul 2016]

Uniprot Description

TAS2R49: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. Belongs to the G-protein coupled receptor T2R family.

Protein type: Receptor, GPCR; GPCR, T2R family; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; sensory perception of taste