Rabbit anti-Human SYTL4 Polyclonal Antibody | anti-Sytl4 antibody

SYTL4, NT (SYTL4, Synaptotagmin-like protein 4, Exophilin-2, Granuphilin)

Cat. Num. AAA647897

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SYTL4; Polyclonal Antibody; NT (SYTL4; Synaptotagmin-like protein 4; Exophilin-2; Granuphilin); Anti -SYTL4; anti-Sytl4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Sytl4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: SYTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-34 amino acids from the N-terminal region of human SYTL4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SYTL4 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SYTL4 antibody detected the SYTL4 protein (arrow).)

Related Product Information for anti-Sytl4 antibody

This gene encodes a member of the synaptotagmin like protein family. Members of this family are characterized by an N-terminal Rab27 binding domain and C-terminal tandem C2 domains. The encoded protein binds specific small Rab GTPases and is involved in intracellular membrane trafficking. This protein binds Rab27 and may be involved in inhibiting dense core vesicle exocytosis. Alternate splicing results in multiple transcript variants that encode the same protein.

Product Categories/Family for anti-Sytl4 antibody

Antibodies; Abs to Syntaxin; Synaptotagmin; Synaptophysin

NCBI and Uniprot Product Information

• NCBI GI #: 17939356
• NCBI GeneID: 140594
• NCBI Accession #: NP_536335.1
• NCBI GenBank Nucleotide #: NM_080410.1
• UniProt Accession #: Q8VHQ7; Q8VHQ6
• Molecular Weight: 75,900 Da
• NCBI Official Full Name: synaptotagmin-like protein 4
• NCBI Official Synonym Full Names: synaptotagmin-like 4
• NCBI Official Symbol: Sytl4
• NCBI Protein Information: synaptotagmin-like protein 4; exophilin-2; granuphilin b; synaptotagmin-like 4 (granuphilin-a)
• UniProt Protein Name: Synaptotagmin-like protein 4
• Protein Family: Synaptotagmin-like protein
• UniProt Gene Name: Sytl4
• UniProt Entry Name: SYTL4_RAT

NCBI Description

binds small GTP-binding protein Rab3 and SNARE-binding protein Munc-18; plays a role in pancreatic beta cell exocytosis; may also be involved in secretory vesicle docking [RGD, Feb 2006]

Uniprot Description

Function: Modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner

By similarity.

Subunit structure: Part of a ternary complex containing STX1A and RAB27A. Can bind both dominant negative and dominant active mutants of RAB27A. Binds STXBP1, RAB3A, RAB8A and RAB27B

By similarity.

Subcellular location: Membrane; Peripheral membrane protein. Cytoplasmic vesicle › secretory vesicle membrane; Peripheral membrane protein. Note: Detected close to the plasma membrane and on secretory granules. In pancreas, interacts with insulin-containing vesicles. Ref.1

Tissue specificity: Detected in insulin-secreting cell lines.

Sequence similarities: Contains 2 C2 domains.Contains 1 FYVE-type zinc finger.Contains 1 RabBD (Rab-binding) domain.