Western Blot (WB)
(Western blot analysis of SYPL2 in human spleen tissue lysate with SYPL2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit SYPL2 Polyclonal Antibody | anti-SYPL2 antibody
SYPL2 Antibody
Gene Names
SYPL2; MG29
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SYPL2 Antibody is affinity chromatography purified via peptide column.
Synonyms
SYPL2; Polyclonal Antibody; SYPL2 Antibody; MG29; Synaptophysin-like protein 2; synaptophysin-like 2; anti-SYPL2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1.
Purity/Purification
SYPL2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
272
Applicable Applications for anti-SYPL2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
SYPL2 antibody can be used for detection of SYPL2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
SYPL2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SYPL.
Buffer
SYPL2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SYPL2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SYPL2 in human spleen tissue lysate with SYPL2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of SYPL2 in human spleen tissue lysate with SYPL2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SYPL2 in human spleen tissue with SYPL2 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SYPL2 in human spleen tissue with SYPL2 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SYPL2 in Human Spleen cells with SYPL2 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SYPL2 in Human Spleen cells with SYPL2 antibody at 20 μg/mL.)
Related Product Information for anti-SYPL2 antibody
SYPL2 Antibody: SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2-null mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,696 Da
NCBI Official Full Name
synaptophysin-like protein 2
NCBI Official Synonym Full Names
synaptophysin-like 2
NCBI Official Symbol
SYPL2
NCBI Official Synonym Symbols
MG29
NCBI Protein Information
synaptophysin-like protein 2; mitsugumin 29; mitsugumin-29
UniProt Protein Name
Synaptophysin-like protein 2
Protein Family
UniProt Gene Name
SYPL2
UniProt Entry Name
SYPL2_HUMAN
Uniprot Description
SYPL2: Involved in communication between the T-tubular and junctional sarcoplasmic reticulum (SR) membranes. Belongs to the synaptophysin/synaptobrevin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1p13.3
Cellular Component: synaptic vesicle; integral to synaptic vesicle membrane
Molecular Function: transporter activity
Biological Process: cellular calcium ion homeostasis; substantia nigra development; transport
Research Articles on SYPL2
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Product Notes
The SYPL2 sypl2 (Catalog #AAA151588) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SYPL2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SYPL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). SYPL2 antibody can be used for detection of SYPL2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the SYPL2 sypl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYPL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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