Mouse anti-Human HUS1 Monoclonal Antibody | anti-HUS1 antibody

HUS1 (Checkpoint Protein HUS1, hHUS1) (AP)

Cat. Num. AAA6131784

• Gene Names: HUS1; hHUS1
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HUS1; Monoclonal Antibody; HUS1 (Checkpoint Protein HUS1; hHUS1) (AP); anti-HUS1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2B12
• Specificity: Recognizes human HUS1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HUS1 antibody: ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: Sandwich ELISA: The detection limit is ~0.1ng/ml as a capture antibody; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-280 from human HUS1 (AAH07013) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunoprecipitation (IP)

(Immunoprecipitation of HUS1 transfected lysate using HUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HUS1 rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged HUS1 is 0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-HUS1 antibody

Antibodies; DNA Repair/Damage

NCBI and Uniprot Product Information

• NCBI GI #: 13937823
• NCBI GeneID: 3364
• Molecular Weight: 29,287 Da
• NCBI Official Full Name: Homo sapiens HUS1 checkpoint homolog (S. pombe), mRNA
• NCBI Official Synonym Full Names: HUS1 checkpoint clamp component
• NCBI Official Symbol: HUS1
• NCBI Official Synonym Symbols: hHUS1
• NCBI Protein Information: checkpoint protein HUS1
• Protein Family: Checkpoint protein

NCBI Description

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]