Rabbit SYNPO Polyclonal Antibody | anti-SYNPO antibody

SYNPO Antibody

Cat. Num. AAA151009

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: SYNPO Antibody is affinity chromatography purified via peptide column.
• Synonyms: SYNPO; Polyclonal Antibody; SYNPO Antibody; KIAA1029; Synaptopodin; synaptopodin; anti-SYNPO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SYNPO Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 929

• Applicable Applications for anti-SYNPO antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: SYNPO antibody can be used for detection of SYNPO by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SYNPO antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SYNPO.
• Buffer: SYNPO Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SYNPO antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SYNPO in rat brain tissue lysate with SYNPO antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of SYNPO in human brain tissue with SYNPO antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SYNPO in human brain tissue with SYNPO antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SYNPO in mouse brain tissue with SYNPO antibody at 20 μg/mL.Red: SYNPO Antibody (6049)Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of SYNPO in mouse brain tissue with SYNPO antibody at 5 μg/mL.)

Related Product Information for anti-SYNPO antibody

SYNPO Antibody: SYNPO, also known as synaptopodin, is an actin-associated protein in telencephalic dendrites and renal podocytes. SYNPO is tightly associated with the dendritic spine apparatus, and mice lacking the SYNPO gene lack the apparatus. These SYNPO-null mice also demonstrate an impaired ability to express long-term potentiation as well as deficits in spatial memory tasks, indicating that SYNPO is involved in the regulation of synaptic plasticity. Recent studies suggest that SYNPO is linked to neuronal calcium stores and plays a role in the calcium store-associated ability of neurons to undergo long-term plasticity.

NCBI and Uniprot Product Information

• NCBI GI #: 261278297
• NCBI GeneID: 11346
• NCBI Accession #: NP_001159680
• NCBI GenBank Nucleotide #: NM_001166208.1
• UniProt Accession #: Q8N3V7; O15271; Q9UPX1; A5PKZ8; D3DQG8
• Molecular Weight: 73,667 Da
• NCBI Official Full Name: synaptopodin isoform C
• NCBI Official Synonym Full Names: synaptopodin
• NCBI Official Symbol: SYNPO
• NCBI Protein Information: synaptopodin
• UniProt Protein Name: Synaptopodin
• Protein Family: Synaptopodin
• UniProt Gene Name: SYNPO
• UniProt Synonym Gene Names: KIAA1029
• UniProt Entry Name: SYNPO_HUMAN

NCBI Description

Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes (Mundel et al., 1997 [PubMed 9314539]).[supplied by OMIM, Mar 2008]

Uniprot Description

SYNPO: an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.

Protein type: Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: postsynaptic membrane; tight junction; postsynaptic density; cytoplasm; dendritic spine; stress fiber; perikaryon; actin cytoskeleton

Molecular Function: protein binding; actin binding

Biological Process: regulation of stress fiber formation; positive regulation of actin filament bundle formation