Western Blot (WB)
(Anti- Synaptopodin antibody, Western blottingAll lanes: Anti Synaptopodin at 0.5ug/mlLane 1: Mosue Brain Tissue Lysate at 50ugLane 2: U87 Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugPredicted bind size: 99KDObserved bind size: 99KD)
anti-Human, Mouse Synaptopodin Polyclonal Antibody | anti-SYNPO antibody
Anti-Synaptopodin Antibody
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Synaptopodin; Polyclonal Antibody; Anti-Synaptopodin Antibody; KIAA1029; SYNPO; SYNPO_HUMAN; synaptopodin; anti-SYNPO antibody
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
685
Applicable Applications for anti-SYNPO antibody
Western Blot (WB)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptopodin(629-658aa EKPKVTPNPDLLDLVQTADEKRRQRDQGEV), different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- Synaptopodin antibody, Western blottingAll lanes: Anti Synaptopodin at 0.5ug/mlLane 1: Mosue Brain Tissue Lysate at 50ugLane 2: U87 Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugPredicted bind size: 99KDObserved bind size: 99KD)
Western Blot (WB)
(Anti- Synaptopodin antibody, Western blottingAll lanes: Anti Synaptopodin at 0.5ug/mlLane 1: Mosue Brain Tissue Lysate at 50ugLane 2: U87 Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugPredicted bind size: 99KDObserved bind size: 99KD)
Related Product Information for anti-SYNPO antibody
Description: Rabbit IgG polyclonal antibody for Synaptopodin(SYNPO) detection. Tested with WB in Human;Mouse.
Background: Synaptopodin is also known as SYNPO. The spine apparatus (SA) is a specialized form of endoplasmic reticulum (ER) that is found in a subpopulation of dendritic spines in central neurons. The SA consists of a series of stacked discs that are thought to be connected to each other and to the dendritic system of ER-tubules. The actin binding protein synaptopodin (which has originally been described in podocytes of the kidney) is an essential component of the SA. Mice that lack the gene for synaptopodin do not form a spine apparatus. The SA is believed to play a critical role in learning and memory. In summary, an important function of the spine apparatus is the regulation of plasticity at individual synapses, a process known as metaplasticity. The International Radiation Hybrid Mapping Consortium mapped the SYNPO gene to chromosome 5.
Background: Synaptopodin is also known as SYNPO. The spine apparatus (SA) is a specialized form of endoplasmic reticulum (ER) that is found in a subpopulation of dendritic spines in central neurons. The SA consists of a series of stacked discs that are thought to be connected to each other and to the dendritic system of ER-tubules. The actin binding protein synaptopodin (which has originally been described in podocytes of the kidney) is an essential component of the SA. Mice that lack the gene for synaptopodin do not form a spine apparatus. The SA is believed to play a critical role in learning and memory. In summary, an important function of the spine apparatus is the regulation of plasticity at individual synapses, a process known as metaplasticity. The International Radiation Hybrid Mapping Consortium mapped the SYNPO gene to chromosome 5.
References
1. Cooney; Hurlburt, JL; Selig, DK; Harris, KM; Fiala, JC (2002). "Endosomal compartments serve multiple hippocampal dendritic spines from a widespread rather than a local store of recycling membrane". The Journal of neuroscience : the official journal of the Society for Neuroscience 22 (6): 2215-24. 2. Deller; Merten, T; Roth, SU; Mundel, P; Frotscher, M (2000). "Actin-associated protein synaptopodin in the rat hippocampal formation: localization in the spine neck and close association with the spine apparatus of principal neurons". The Journal of Comparative Neurology 418 (2): 164-81 3. Gray (1959). "Electron microscopy of synaptic contacts on dendrite spines of the cerebral cortex". Nature 183 (4675): 1592-3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,667 Da
NCBI Official Full Name
synaptopodin isoform B
NCBI Official Synonym Full Names
synaptopodin
NCBI Official Symbol
SYNPO
NCBI Protein Information
synaptopodin
UniProt Protein Name
Synaptopodin
Protein Family
UniProt Gene Name
SYNPO
UniProt Synonym Gene Names
KIAA1029
UniProt Entry Name
SYNPO_HUMAN
NCBI Description
Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes (Mundel et al., 1997 [PubMed 9314539]).[supplied by OMIM, Mar 2008]
Uniprot Description
SYNPO: an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
Protein type: Actin-binding; Cytoskeletal
Chromosomal Location of Human Ortholog: 5q33.1
Cellular Component: actin cytoskeleton; cytoplasm; dendritic spine; perikaryon; postsynaptic density; postsynaptic membrane; stress fiber; tight junction
Molecular Function: actin binding; protein binding
Biological Process: positive regulation of actin filament bundle formation; regulation of stress fiber formation
Research Articles on SYNPO
Similar Products
Product Notes
The SYNPO synpo (Catalog #AAA178136) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Synaptopodin Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Synaptopodin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the SYNPO synpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptopodin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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