Rabbit anti-Human Synaptotagmin I/II Polyclonal Antibody | anti-SYT2 antibody

Synaptotagmin I/II (Syt)

Cat. Num. AAA620319

• Gene Names: SYT2; SytII; FLJ42519
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Synaptotagmin I/II; Polyclonal Antibody; Synaptotagmin I/II (Syt); Anti -Synaptotagmin I/II (Syt); anti-SYT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total Synaptotagmin I/II protein.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-SYT2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:1,000
• Immunogen: Synthesized non-phosphopeptide derived from human Synaptotagmin I/II around the phosphorylation site of threonine 202/199 (R-K-TP-L-N).
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extract from Hela cells using Rabbit Anti-Synaptotagmin I/II (Ab-202/199) Polyclonal Antibody (GenScript, A01170, lane 1 and 2)

Related Product Information for anti-SYT2 antibody

Synaptotagmins (Syt) are membrane-trafficking proteins characterized by an N-terminal transmembrane region, a variable linker and two C-terminal C2-domains, C2A and C2B. At least twelve synaptotagamins are expressed in vertebrates and can be found in both vesicular and plasma membranes. Synaptotagamins are membrane-bound Ca2+-sensor proteins that bind Ca2+ with low affinity. Although all Syts bind Ca2+ with the same cooperativity and participate in Ca2+ dependent phospholipid binding they have distinct Ca2+-binding properties and affinities (3). Two broad subfamilies have been proposed based on expression pattern. Syt 1-5, 10, 11 and Srg 1 are primarily expressed in nervous and other endocrine tissues while Syt 6-9 are ubiquitously expressed (4). The calcium-dependent, lipid-binding properties of Syt 1 suggest its involvement in lipid rearrangements required during synaptic vesicle fusion (3). The three cytosolic loops that form the Ca2+ binding pocket in the C2A domain insert into phospholipid bilayers upon Ca2+ binding. Ca2+ binding also triggers oligimerization of Syt via the C2B domain (4). Plasma membrane Syts have higher Ca2+ affinity than vesicular Syts and are are proposed to be more important for endocrine exocytosis, while vesicular Syts are thought to be more important in fast synaptic exocytosis. The distinct properties, combinantions and relative abundance of various Syts may influence the Ca2+ responses of synapses (3). Syts have also been shown to interact with neural proteins in the absence of calcium, suggesting roles in presynaptic functions. These interactions include binding to calcium channels, sodium channels, the clatherin adapter AP-2, the stoned endocytotic proteins, beta-SNAP, neurexins, calmodulin, inositol polyphosphates, SYNCRIP and polyphosphoinositides (4).

Product Categories/Family for anti-SYT2 antibody

Antibodies; Abs to Syntaxin; Synaptotagmin; Synaptophysin

NCBI and Uniprot Product Information

• NCBI GI #: 71680826
• NCBI GeneID: 127833
• UniProt Accession #: Q8N9I0; Q496K5; Q8NBE5
• Molecular Weight: 46,872 Da
• NCBI Official Full Name: Synaptotagmin II
• NCBI Official Synonym Full Names: synaptotagmin II
• NCBI Official Symbol: SYT2
• NCBI Official Synonym Symbols: SytII; FLJ42519
• NCBI Protein Information: synaptotagmin-2; synaptotagmin 2; OTTHUMP00000038803; OTTHUMP00000038804
• UniProt Protein Name: Synaptotagmin-2
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT2
• UniProt Entry Name: SYT2_HUMAN

NCBI Description

Synaptotagmins, like SYT2, are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (Hilbush and Morgan, 1994 [PubMed 8058779]).[supplied by OMIM]

Uniprot Description

SYT2: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. Belongs to the synaptotagmin family.

Protein type: Membrane protein, integral; Lipid-binding; Calcium-binding; Vesicle

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: intracellular membrane-bound organelle; synaptic vesicle membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding

Biological Process: transport; pathogenesis

Disease: Myasthenic Syndrome, Presynaptic, Congenital, With Or Without Motor Neuropathy