Rabbit anti-Human, Mouse SUZ12 Polyclonal Antibody | anti-SUZ12 antibody

Anti-SUZ12 Antibody

Cat. Num. AAA1752836

• Gene Names: SUZ12; CHET9; JJAZ1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen Affinity Purified
• Synonyms: SUZ12; Polyclonal Antibody; Anti-SUZ12 Antibody; Polycomb protein SUZ12; Chromatin precipitated E2F target 9 protein ChET 9 protein; Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog; CHET9; JJAZ1; KIAA0160; anti-SUZ12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 688

• Applicable Applications for anti-SUZ12 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/mL; IHC-P: 0.5-1ug/mL; ICC: 0.5-1ug/mL; FC/FACS: 1-3mug/1x106 cells; ELISA: 0.1-0.5ug/mL; Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested.
• Immunogen: E. coli-derived human SUZ12 recombinant protein (Position: K72-K483).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500mug/ml.
• Relevant Detection Systems: MyBioSource recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P), and ICC.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, store at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-SUZ12 antibody

Description: Rabbit IgG polyclonal antibody for SUZ12 detection. Tested with WB, IHC-P, ICC, FCM, Direct ELISA in Human; Mouse.

Background: Polycomb protein SUZ12 is a protein that in humans is encoded by the SUZ12 gene. It is mapped to 17q11.2. This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

References

1. Nagase T, Seki N, Tanaka A, Ishikawa K, Nomura N (August 1995). "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1". DNA Research. 2 (4): 167-74, 199-210. 2. Koontz JI, Soreng AL, Nucci M, Kuo FC, Pauwels P, van Den Berghe H, Dal Cin P, Fletcher JA, Sklar J (May 2001). "Frequent fusion of the JAZF1 and JJAZ1 genes in endometrial stromal tumors". Proceedings of the National Academy of Sciences of the United States of America. 98 (11): 6348-53. 3. Ross PJ, Ragina NP, Rodriguez RM, Iager AE, Siripattarapravat K, Lopez-Corrales N, Cibelli JB (December 2008). "Polycomb gene expression and histone H3 lysine 27 trimethylation changes during bovine preimplantation development". Reproduction. 136 (6): 777-85.

NCBI and Uniprot Product Information

• NCBI GI #: 119600664
• NCBI GeneID: 23512
• UniProt Accession #: Q15022
• NCBI Official Full Name: suppressor of zeste 12 homolog (Drosophila)
• NCBI Official Synonym Full Names: SUZ12 polycomb repressive complex 2 subunit
• NCBI Official Symbol: SUZ12
• NCBI Official Synonym Symbols: CHET9; JJAZ1
• NCBI Protein Information: polycomb protein SUZ12
• UniProt Protein Name: Polycomb protein SUZ12
• Protein Family: Polycomb protein
• UniProt Gene Name: SUZ12
• UniProt Synonym Gene Names: CHET9; JJAZ1; KIAA0160; ChET 9 protein
• UniProt Entry Name: SUZ12_HUMAN

NCBI Description

This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009]

Uniprot Description

SUZ12: a zinc finger protein. A breakpoint translocation of the cognate gene with JAZF1 has been reported in endometrial stromal sarcoma.

Protein type: Oncoprotein; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; sex chromatin; ESC/E(Z) complex; nucleolus; nucleus

Molecular Function: histone methyltransferase activity; protein binding; sequence-specific DNA binding; RNA binding; metal ion binding; chromatin binding; methylated histone residue binding

Biological Process: histone methylation; negative regulation of cell differentiation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; histone ubiquitination; positive regulation of cell proliferation; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic