Rabbit anti-Human, Mouse Surfactant Protein A Polyclonal Antibody | anti-SP-A antibody

Surfactant Protein A (SP-A) (HRP)

Cat. Num. AAA6501684

• Gene Names: SFTPA1; SPA; PSAP; PSPA; SP-A; SPA1; PSP-A; SFTP1; SP-A1; COLEC4; SFTPA1B; SP-A1 beta; SP-A1 delta; SP-A1 gamma; SP-A1 epsilon
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Surfactant Protein A; Polyclonal Antibody; Surfactant Protein A (SP-A) (HRP); anti-SP-A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the C-type lectin domain of human Surfactant Protein A. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 248

• Applicable Applications for anti-SP-A antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 0.5ug/ml. Detected SP-A in 10ug of human lung tissue lysate, band at ~35kD.; IHC: 1:200; Applications are based on unconjugated antibody.
• Immunogen: Linear peptide corresponding to the C-type lectin domain of human Surfactant Protein A, KLH-conjugated (Accession# NP_005402). Species sequence homology: primate
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB Analysis: Representative lot data. Human lung tissue lysate was probed with S8400 Anti-Surfactant Protein A (0.5 ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Surfactant Protein A (~35kD))

Immunohistochemistry (IHC)

(IHC Analysis:Representative lot data. Formalin Fixed Paraffin Embedded (FFPE) human lung tissue was processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:200 dilution of S8400. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Apical epithelial membrane staining was observed in human lung tissue)

Product Categories/Family for anti-SP-A antibody

Antibodies; Proteins

References

1. Stahlman, M.T., et al., Pediatr. Res. 31: 364-371 (1992). 2. Khoor, A., et al., J. Histochem. Cytochem. 42: 1187-1199 (1994). 3. Coalson, J.J., et al., Am. J. Respir. Crit. Care Med. 151: 854-866 (1995). 4. Jennings, V.M., et al., Am. J. Respir. Cell Mol. Biol. 13: 297-306 (1995). 5. Vorbroker, D.K., et al., Am. J. Physiol. 268: (Lung. Cell Mol. Physiol. 13) L647-L656 (1995). 6. Wert, S.E., et al., Am. J. Respir. Crit. Care Med. 149: A244 (1994). 7. Wert, S.E., et al., Pediatr. Res. 39: 355A (1996). 8. Wert, S.E., et al., Pediatr. Res. 37: 356A (1995). 9. Morotti, R.A., et al., Human Pathology 30: 618-625 (1999). 10. Nogee, L.M., et al., Am. J. Respir. Crit. Care Med. 161: 973-981 (2000). 11. Shi, S-R., et al., J. Histochem. Cytochem. 39: 741-748 (1991). 12. Gown, A.M. & Leong, A., Appl. Immunohistochem. 14: 267-274 (1993). 13. Zhou, L., et al., Dev. Biol. 175: 227-238 (1996). 14. Zhou, L., et al., J. Histochem. Cytochem. 44: 1183-1193 (1996). 15. Jain-Vora, S., et al., Am. J. Respir. Cell Mol. Biol. 17: 1-11 (1997). 16. Zsengeller, Z.K., et al., Hum. Gene Ther. 8: 1331-1344 (1997). 17. Tokieda, K., et al., Am. J. Respir. Cell and Mol. Biol. 21: 463-472 (1999).9).

NCBI and Uniprot Product Information

• NCBI GI #: 38888175
• NCBI GeneID: 653509
• NCBI Accession #: NP_005402
• NCBI GenBank Nucleotide #: NP_005402.3
• UniProt Accession #: Q8IWL2
• NCBI Official Full Name: pulmonary surfactant-associated protein A1 isoform 1
• NCBI Official Synonym Full Names: surfactant protein A1
• NCBI Official Symbol: SFTPA1
• NCBI Official Synonym Symbols: SPA; PSAP; PSPA; SP-A; SPA1; PSP-A; SFTP1; SP-A1; COLEC4; SFTPA1B; SP-A1 beta; SP-A1 delta; SP-A1 gamma; SP-A1 epsilon
• NCBI Protein Information: pulmonary surfactant-associated protein A1
• UniProt Protein Name: Pulmonary surfactant-associated protein A1
• UniProt Gene Name: SFTPA1
• UniProt Synonym Gene Names: COLEC4; PSAP; SFTP1; SFTPA; SFTPA1B; PSP-A; PSPA; SP-A; SP-A1
• UniProt Entry Name: SFTA1_HUMAN

NCBI Description

This gene encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins. The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms. This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens. Mutations in this gene are associated with idiopathic pulmonary fibrosis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]