Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) Standard Curve.)
Mouse Cytosolic Fe-S Cluster Assembly Factor NUBP2 (NUBP2) ELISA Kit | NUBP2 elisa kit
Mouse Cytosolic Fe-S Cluster Assembly Factor NUBP2 (NUBP2) ELISA Kit
Gene Names
Nubp2; D17Wsu11e
Reactivity
Mouse
Synonyms
Cytosolic Fe-S Cluster Assembly Factor NUBP2 (NUBP2); Mouse Cytosolic Fe-S Cluster Assembly Factor NUBP2 (NUBP2) ELISA Kit; NUBP2; CFD1; NUBP1; C447E6.1 (nucleotide binding protein 1 (E.coli MinD like)); homolog of yeast cytosolic Fe-S cluster deficient 1; nucleotide binding protein 2 (E.coli MinD like); NUBP2 elisa kit
Reactivity
Mouse
Specificity
Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NUBP2. No significant cross-reactivity or interference between Mouse NUBP2 and analogues was observed.
Sequence Length
275
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) Standard Curve.)
Related Product Information for NUBP2 elisa kit
Description: This Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate NUBP2 in samples. An antibody specific for NUBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NUBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NUBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NUBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins. Nakashima et al. (1999) cloned mouse Nubp2 and used the murine sequence as a probe in EST database analysis, followed by screening a human teratoma cDNA library to clone full-length human NUBP2. The 271-amino acid human NUBP2 protein shares 72.6% amino acid similarity with mouse Nubp2. Northern blot analysis detected a 1.4-kb NUBP2 transcript with ubiquitous expression in all human adult and fetal tissues tested, with highest expression in adult skeletal muscle. NUBP2 contains conserved an ATP/GTP binding motif A (P-loop), an ATP/GTP binding motif A-prime, and NUBP/MRP alpha and beta motifs. NUBP2 lacks an additional N-terminal sequence with 4 cysteine residues that is present in NUBP1.
Background: NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins. Nakashima et al. (1999) cloned mouse Nubp2 and used the murine sequence as a probe in EST database analysis, followed by screening a human teratoma cDNA library to clone full-length human NUBP2. The 271-amino acid human NUBP2 protein shares 72.6% amino acid similarity with mouse Nubp2. Northern blot analysis detected a 1.4-kb NUBP2 transcript with ubiquitous expression in all human adult and fetal tissues tested, with highest expression in adult skeletal muscle. NUBP2 contains conserved an ATP/GTP binding motif A (P-loop), an ATP/GTP binding motif A-prime, and NUBP/MRP alpha and beta motifs. NUBP2 lacks an additional N-terminal sequence with 4 cysteine residues that is present in NUBP1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytosolic Fe-S cluster assembly factor NUBP2 isoform 1
NCBI Official Synonym Full Names
nucleotide binding protein 2
NCBI Official Symbol
Nubp2
NCBI Official Synonym Symbols
D17Wsu11e
NCBI Protein Information
cytosolic Fe-S cluster assembly factor NUBP2
UniProt Protein Name
Cytosolic Fe-S cluster assembly factor NUBP2
Protein Family
UniProt Gene Name
Nubp2
UniProt Synonym Gene Names
NBP 2
UniProt Entry Name
NUBP2_MOUSE
Research Articles on NUBP2
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Product Notes
The Mouse NUBP2 nubp2 (Catalog #AAA9718067) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718067 ELISA Kit recognizes Mouse NUBP2. It is sometimes possible for the material contained within the vial of "Cytosolic Fe-S Cluster Assembly Factor NUBP2 (NUBP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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