Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SUPT5H antibody at 1:1000 dilution.)
Rabbit anti-Human, Mouse SUPT5H Polyclonal Antibody | anti-SUPT5H antibody
SUPT5H Polyclonal Antibody
Gene Names
SUPT5H; SPT5; SPT5H; Tat-CT1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SUPT5H; Polyclonal Antibody; SUPT5H Polyclonal Antibody; SPT5; SPT5H; Tat-CT1; anti-SUPT5H antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SUPT5H polyclonal antibody detects endogenous levels of SUPT5H protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1087
Applicable Applications for anti-SUPT5H antibody
Western Blot (WB)
Application Notes
WB: 1:200-1:2000
Immunogen
Recombinant protein of human SUPT5H
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SUPT5H antibody at 1:1000 dilution.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using SUPT5H antibody at 1:1000 dilution.)
Related Product Information for anti-SUPT5H antibody
SPT4 (also designated Suppressor of Ty4 and p14) and SPT5 (also designated DSIF p160) are highly conserved proteins from yeast to humans. Nuclear SPT4 and SPT5 are involved in both DRB (5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the activation of transcriptional elongation by the HIV-1 protein Tat. SPT4 binds SPT5 to form the DSIF (DRB-sensitivity-inducing factor) complex, which binds RNA polymerase II and directly regulates elongation. However, SPT5 protein in mitotic HeLa cells migrates more slowly on SDS-PAGE than does SPT5 isolated from interphase cells, as a result of enhanced SPT5 phosphorylation. The C-terminal CTR1 domain of SPT5 is the substrate for P-TEFb phosphorylation, which is critical for SPT5 function as a regulator of transcriptional elongation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
170kDa
NCBI Official Full Name
Transcription elongation factor SPT5
NCBI Official Synonym Full Names
SPT5 homolog, DSIF elongation factor subunit
NCBI Official Symbol
SUPT5H
NCBI Official Synonym Symbols
SPT5; SPT5H; Tat-CT1
NCBI Protein Information
transcription elongation factor SPT5
UniProt Protein Name
Transcription elongation factor SPT5
UniProt Gene Name
SUPT5H
UniProt Synonym Gene Names
SPT5; SPT5H
UniProt Entry Name
SPT5H_HUMAN
Research Articles on SUPT5H
Similar Products
Product Notes
The SUPT5H supt5h (Catalog #AAA3009770) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SUPT5H Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SUPT5H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200-1:2000. Researchers should empirically determine the suitability of the SUPT5H supt5h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUPT5H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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