Rabbit anti-Human SUMO4 Polyclonal Antibody | anti-SUMO4 antibody

SUMO4, ID (Small Ubiquitin-related Modifier 4, Small Ubiquitin-like Protein 4, SUMO-4, SMT3H4) (HRP)

Cat. Num. AAA6501623

• Gene Names: SUMO4; IDDM5; SMT3H4; SUMO-4; dJ281H8.4
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SUMO4; Polyclonal Antibody; ID (Small Ubiquitin-related Modifier 4; Small Ubiquitin-like Protein 4; SUMO-4; SMT3H4) (HRP); anti-SUMO4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SUMO4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 95

• Applicable Applications for anti-SUMO4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SUMO4 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of SUMO4 Antibody (Center) in HepG2 cell line lysate. SUMO4(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of SUMO4 (arrow) using rabbit polyclonal SUMO4 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO4 gene.)

Product Categories/Family for anti-SUMO4 antibody

Antibodies; Ubiquitin

References

1. Park, Y., et al. Nat. Genet. 37(2), 112 (2005). 2. Guo, D., et al. Nat. Genet. 36(8), 837-841 (2004). 3. Bohren, K.M., et al. J. Biol. Chem. 279(26), 27233-27238 (2004). 4. Yang, S.H., et al., Mol. Cell 13(4):611-617 (2004). 5. Bailey, D., et al., J. Biol. Chem. 279(1):692-703 (2004). 6. Ling, Y., et al., Nucleic Acids Res. 32(2):598-610 (2004). 7. Pountney, D.L., et al., Exp. Neurol. 184(1):436-446 (2003). 8. Ohshima, T., et al., J. Biol. Chem. 278(51):50833-50842 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 50400081
• NCBI GeneID: 387082
• NCBI Accession #: NP_001002255
• NCBI GenBank Nucleotide #: NP_001002255.1
• UniProt Accession #: Q6EEV6
• Molecular Weight: 10.653
• NCBI Official Full Name: small ubiquitin-related modifier 4
• NCBI Official Synonym Full Names: small ubiquitin like modifier 4
• NCBI Official Symbol: SUMO4
• NCBI Official Synonym Symbols: IDDM5; SMT3H4; SUMO-4; dJ281H8.4
• NCBI Protein Information: small ubiquitin-related modifier 4
• UniProt Protein Name: Small ubiquitin-related modifier 4
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO4
• UniProt Synonym Gene Names: SMT3H4; SUMO-4
• UniProt Entry Name: SUMO4_HUMAN

NCBI Description

This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq, Jul 2008]

Uniprot Description

SUMO4: Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. Belongs to the ubiquitin family. SUMO subfamily.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: nucleus

Biological Process: protein sumoylation

Disease: Diabetes Mellitus, Insulin-dependent, 5