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Immunohistochemistry (IHC) (Anti-SUMO2/SUMO3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Rabbit anti-Human, Mouse SUMO2 + SUMO3 Polyclonal Antibody

Rabbit Polyclonal to Human SUMO2 + SUMO3

Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
SUMO2 + SUMO3; Polyclonal Antibody; Rabbit Polyclonal to Human SUMO2 + SUMO3; Anti-SUMO2 + SUMO3 Antibody (C-Terminus) IHC-plus; Human SUMO2 + SUMO3; anti-SUMO2 + SUMO3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.09% sodium azide
Applicable Applications for anti-SUMO2 + SUMO3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (5 ug/ml), WB (1:1000)
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide selected within C-terminal region of human SUMO2/3.
Immunogen Type
Synthetic peptide
Immunogen
SUMO2 + SUMO3 antibody was raised against kLH conjugated synthetic peptide selected within C-terminal region of human SUMO2/3.
Antigen Modification
C-Terminus
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-SUMO2/SUMO3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-SUMO2/SUMO3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SUMO2/SUMO3 antibody IHC of human cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-SUMO2/SUMO3 antibody IHC of human cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(COS-7 cells were transfected for 24 hrs with a plasmid expressing DYKDDDDK-ERM (left panels) or DYKDDDDK-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by Western blot with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-DYKDDDDK antibody followed by WB with AP1222a SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-DYKDDDDK antibody followed by WB with SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.)

Western Blot (WB) (COS-7 cells were transfected for 24 hrs with a plasmid expressing DYKDDDDK-ERM (left panels) or DYKDDDDK-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by Western blot with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-DYKDDDDK antibody followed by WB with AP1222a SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-DYKDDDDK antibody followed by WB with SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.)

Western Blot (WB)

(The anti-SUMO2/3 C-term Pab is used in Western blot to detect SUMO2/3 in HeLa cell lysate.)

Western Blot (WB) (The anti-SUMO2/3 C-term Pab is used in Western blot to detect SUMO2/3 in HeLa cell lysate.)

Western Blot (WB)

(Western blot of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Q65). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.)

Western Blot (WB) (Western blot of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Q65). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.)

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Product Notes

The SUMO2 + SUMO3 (Catalog #AAA247813) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human SUMO2 + SUMO3 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SUMO2 + SUMO3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (5 ug/ml), WB (1:1000). Researchers should empirically determine the suitability of the SUMO2 + SUMO3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUMO2 + SUMO3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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