Rabbit anti-Human, Mouse SULT2A Polyclonal Antibody | anti-SULT2A antibody

SULT2A, CT (Bile Salt Sulfotransferase, Hydroxysteroid Sulfotransferase, HST, Dehydroepiandrosterone Sulfotransferase, DHEA-ST, Sulfotransferase 2A1, ST2A1, ST2,ST2A3, STD) (Azide free) (HRP)

Cat. Num. AAA6501447

• Gene Names: SULT2A1; HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SULT2A; Polyclonal Antibody; CT (Bile Salt Sulfotransferase; Hydroxysteroid Sulfotransferase; HST; Dehydroepiandrosterone Sulfotransferase; DHEA-ST; Sulfotransferase 2A1; ST2A1; ST2; ST2A3; STD) (Azide free) (HRP); anti-SULT2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SULT2A. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SULT2A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SULT2A (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-SULT2A Pab is used in Western blot to detect SULT2A in mouse kidney tissue lysate.)

Product Categories/Family for anti-SULT2A antibody

Antibodies; Enzymes; Sulfotransferase

References

1. Otterness, D. M., et al. Molec. Pharm. 41: 865-872 (1992). 2. Kong, A.-N. T., et al. Biochem. Biophys. Res. Commun. 187: 448-454 (1992). 3. Comer, K. A., et al. Biochem. J. 289: 233-240 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 29540545
• NCBI GeneID: 6822
• NCBI Accession #: NP_003158
• NCBI GenBank Nucleotide #: NM_003167.3
• UniProt Accession #: Q06520
• Molecular Weight: 35.9 kDa (305aa), confirmed by MALDI-TOF
• NCBI Official Full Name: bile salt sulfotransferase
• NCBI Official Synonym Full Names: sulfotransferase family 2A member 1
• NCBI Official Symbol: SULT2A1
• NCBI Official Synonym Symbols: HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• NCBI Protein Information: bile salt sulfotransferase
• UniProt Protein Name: Bile salt sulfotransferase
• UniProt Gene Name: SULT2A1
• UniProt Synonym Gene Names: HST; STD; DHEA-ST; HST; ST2A1
• UniProt Entry Name: ST2A1_HUMAN

NCBI Description

This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome. [provided by RefSeq, Mar 2010]

Uniprot Description

SULT2A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Belongs to the sulfotransferase 1 family.

Protein type: EC 2.8.2.14; Transferase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; protein binding; bile-salt sulfotransferase activity

Biological Process: steroid metabolic process; bile acid catabolic process; xenobiotic metabolic process; digestion; sulfation; cellular lipid metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process