Western Blot (WB)
((0.3ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SULF2 Polyclonal Antibody | anti-SULF2 antibody
Goat anti-SULF2 Antibody
Western Blot: Approx 90kDa band observed in Human Ovary lysates (calculated MW of 98.2kDa according to NP_940998.1). Recommended concentration: 0.3-1ug/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Western Blot (WB)
((0.3ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]
Uniprot Description
SULF2: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Secreted, signal peptide; EC 3.1.6.-; Secreted
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: cell surface; endoplasmic reticulum; extracellular space; Golgi stack; plasma membrane
Molecular Function: arylsulfatase activity; calcium ion binding; N-acetylglucosamine-6-sulfatase activity
Biological Process: cartilage development; chondrocyte development; embryonic skeletal development; esophagus smooth muscle contraction; glomerular basement membrane development; glomerular filtration; heparan sulfate proteoglycan metabolic process; kidney development; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of Wnt receptor signaling pathway; response to wounding
Research Articles on SULF2
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Product Notes
The SULF2 sulf2 (Catalog #AAA422166) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SULF2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SULF2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 90kDa band observed in Human Ovary lysates (calculated MW of 98.2kDa according to NP_940998.1). Recommended concentration: 0.3-1ug/ml. An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. Researchers should empirically determine the suitability of the SULF2 sulf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DGRVYHVGLG DAAQPR. It is sometimes possible for the material contained within the vial of "SULF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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