Western Blot (WB)
(SUGT1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the SUGT1 antibody detected the SUGT1 protein (arrow).)
Rabbit anti-Human SUGT1 Polyclonal Antibody | anti-Sugt1 antibody
SUGT1, CT (SUGT1, Suppressor of G2 allele of SKP1 homolog, Protein 40-6-3, Sgt1)
Gene Names
Sugt1; Aa1114
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
SUGT1; Polyclonal Antibody; CT (SUGT1; Suppressor of G2 allele of SKP1 homolog; Protein 40-6-3; Sgt1); Anti -SUGT1; anti-Sugt1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Sugt1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
SUGT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 329-356 amino acids selected from the C-terminal region of human SUGT1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(SUGT1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the SUGT1 antibody detected the SUGT1 protein (arrow).)
Western Blot (WB)
(SUGT1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the SUGT1 antibody detected the SUGT1 protein (arrow).)
Related Product Information for anti-Sugt1 antibody
This gene is homologous to the yeast gene SGT1, which encodes a protein involved in kinetochore function and required for the G1/S and G2/M transitions. Complementation studies suggest that the human protein has similar functions. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-Sugt1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,091 Da
NCBI Official Full Name
suppressor of G2 allele of SKP1 homolog
NCBI Official Synonym Full Names
SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
NCBI Official Symbol
Sugt1
NCBI Official Synonym Symbols
Aa1114
NCBI Protein Information
suppressor of G2 allele of SKP1 homolog
UniProt Protein Name
Suppressor of G2 allele of SKP1 homolog
UniProt Gene Name
Sugt1
UniProt Entry Name
SUGT1_RAT
Uniprot Description
Function: May play a role in ubiquitination and subsequent proteasomal degradation of target proteins.
Subunit structure: Probably associates with SCF (SKP1-CUL1-F-box protein) complex through interaction with SKP1. Interacts with S100A6. Interacts with HSP90
By similarity.
Subcellular location: Cytoplasm
By similarity. Nucleus
By similarity. Note: Translocates the to nucleus upon heat shock, requiring S100A6
By similarity.
Domain: The CS domain mediates interaction with HSP90
By similarity.
Post-translational modification: Phosphorylated at Ser-252 and Ser-302, dephosphorylation promotes nuclear translocation, most likely due to disruption of the SUGT1-HSP90 complex
By similarity.
Sequence similarities: Belongs to the SUGT1 family.Contains 1 CS domain.Contains 1 SGS domain.Contains 3 TPR repeats.
Similar Products
Product Notes
The Sugt1 sugt1 (Catalog #AAA646310) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SUGT1, CT (SUGT1, Suppressor of G2 allele of SKP1 homolog, Protein 40-6-3, Sgt1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SUGT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the Sugt1 sugt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUGT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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