Goat anti-Human STXBP1 Polyclonal Antibody | anti-STXBP1 antibody

STXBP1, CT (Syntaxin-binding Protein 1, Protein Unc-18 Homolog, Unc-18-1, Protein Unc-18 Homolog A, Unc-18A, N-Sec1, p67, UNC18A)

Cat. Num. AAA645670

• Gene Names: STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: STXBP1; Polyclonal Antibody; CT (Syntaxin-binding Protein 1; Protein Unc-18 Homolog; Unc-18-1; Protein Unc-18 Homolog A; Unc-18A; N-Sec1; p67; UNC18A); Anti -STXBP1; anti-STXBP1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human STXBP1. Species sequence homology: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-STXBP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:16000; Western Blot: 0.1-0.3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide C-SRVSFEDQAPTME from the C-terminus of human STXBP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

Chemiluminescence

(Antibody (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-STXBP1 antibody

Antibodies; Abs to Syntaxin; Synaptotagmin; Synaptophysin

NCBI and Uniprot Product Information

• NCBI GI #: 73760415
• NCBI GeneID: 6812
• NCBI Accession #: NP_001027392.1
• NCBI GenBank Nucleotide #: NM_001032221.3
• UniProt Accession #: P61764; Q28208; Q62759; Q64320; Q96TG8; B1AM97
• Molecular Weight: 67,569 Da
• NCBI Official Full Name: syntaxin-binding protein 1 isoform b
• NCBI Official Synonym Full Names: syntaxin binding protein 1
• NCBI Official Symbol: STXBP1
• NCBI Official Synonym Symbols: P67; NSEC1; UNC18; RBSEC1; MUNC18-1
• NCBI Protein Information: syntaxin-binding protein 1; N-Sec1; unc-18A; unc18-1; neuronal SEC1; protein unc-18 homolog 1; protein unc-18 homolog A
• UniProt Protein Name: Syntaxin-binding protein 1
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: STXBP1
• UniProt Synonym Gene Names: UNC18A; Unc18-1; Unc-18A
• UniProt Entry Name: STXB1_HUMAN

NCBI Description

This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]

Uniprot Description

Function: May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.

Subunit structure: Binds SYTL4

By similarity. Interacts with STX1A. Ref.6

Subcellular location: Cytoplasm. Membrane; Peripheral membrane protein.

Tissue specificity: Brain and spinal cord. Highly enriched in axons.

Involvement in disease: Epileptic encephalopathy, early infantile, 4 (EIEE4) [MIM:612164]: A severe form of epilepsy characterized by frequent tonic seizures or spasms beginning in infancy with a specific EEG finding of suppression-burst patterns, characterized by high-voltage bursts alternating with almost flat suppression phases. Affected individuals have neonatal or infantile onset of seizures, profound mental retardation, and MRI evidence of brain hypomyelination.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.9

Sequence similarities: Belongs to the STXBP/unc-18/SEC1 family.