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Western Blot (WB) ((0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat STX6 Polyclonal Antibody | anti-STX6 antibody

Goat anti-STX6 Antibody

Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
STX6; Polyclonal Antibody; Goat anti-STX6 Antibody; syntaxin 6; HGNC: 11441; STX6 antibody; syntaxin 6 antibody; HGNC: 11441 antibody; anti-STX6 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QALAERKNRQA
Sequence Length
255
Applicable Applications for anti-STX6 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 30kDa band observed in Human Lymph Node lysates (calculated MW of 29.2kDa according to NP_005810.1). In transfected HEK293 transiently expressing Human STX6 a band of approx.30kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-QALAERKNRQA, from the internal region of the protein sequence according to NP_005810.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(HEK293 overexpressing Human STX6 (RC202951) and probed (mock transfection in first lane), tested by Origene.)

Western Blot (WB) (HEK293 overexpressing Human STX6 (RC202951) and probed (mock transfection in first lane), tested by Origene.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,434 Da
NCBI Official Full Name
syntaxin-6 isoform 1
NCBI Official Synonym Full Names
syntaxin 6
NCBI Official Symbol
STX6
NCBI Protein Information
syntaxin-6
UniProt Protein Name
Syntaxin-6
Protein Family
UniProt Gene Name
STX6
UniProt Entry Name
STX6_HUMAN

Uniprot Description

STX6: Involved in intracellular vesicle trafficking. Binds EEA1. Interacts with VPS45A. Interacts with MARCH2 and MARCH3. Interacts with GOPC. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A. Belongs to the syntaxin family.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: clathrin-coated vesicle; cytosol; early endosome; Golgi apparatus; Golgi membrane; integral to membrane; perinuclear region of cytoplasm; phagocytic vesicle; plasma membrane; SNARE complex; terminal button; trans-Golgi network; trans-Golgi network membrane

Molecular Function: protein binding; SNAP receptor activity; syntaxin binding

Biological Process: endocytic recycling; endosome organization and biogenesis; Golgi vesicle transport; intracellular protein transport; regulation of protein localization; retrograde transport, endosome to Golgi; vesicle docking; vesicle fusion

Research Articles on STX6

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Product Notes

The STX6 stx6 (Catalog #AAA421504) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-STX6 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's STX6 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 30kDa band observed in Human Lymph Node lysates (calculated MW of 29.2kDa according to NP_005810.1). In transfected HEK293 transiently expressing Human STX6 a band of approx.30kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the STX6 stx6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QALAERKNRQ A. It is sometimes possible for the material contained within the vial of "STX6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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