Rabbit anti-Human, Mouse STX5 Polyclonal Antibody | anti-STX5 antibody

STX5, ID (STX5, STX5A, Syntaxin-5) (AP)

Cat. Num. AAA6352368

• Gene Names: STX5; SED5; STX5A
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: STX5; Polyclonal Antibody; ID (STX5; STX5A; Syntaxin-5) (AP); Syntaxin-5; anti-STX5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-STX5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: STX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-209 amino acids from the Central region of human STX5.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(STX5 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the STX5 antibody detected the STX5 protein (arrow).)

Western Blot (WB)

(STX5 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the STX5 antibody detected the STX5 protein (arrow).)

Related Product Information for anti-STX5 antibody

STX5 mediates endoplasmic reticulum to Golgi transport (By similarity).

Product Categories/Family for anti-STX5 antibody

Antibodies; Syntaxin; Synaptotagmin; Synaptophysin

References

Laufman, O., et al. EMBO J. 28(14):2006-2017(2009). Diao, A., et al. J. Biol. Chem. 283(11):6957-6967(2008). Amessou, M., et al. J. Cell. Sci. 120 (PT 8), 1457-1468 (2007): Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). Suga, K., et al. FEBS Lett. 579(20):4226-4234(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 94400932
• NCBI GeneID: 6811
• NCBI Accession #: NP_003155.2
• NCBI GenBank Nucleotide #: NM_003164.4
• UniProt Accession #: Q13190; Q5U0D4; Q7Z3T6; Q9BUG1; B2R8T2; F8W8Q9
• Molecular Weight: 35,474 Da
• NCBI Official Full Name: syntaxin-5 isoform 1
• NCBI Official Synonym Full Names: syntaxin 5
• NCBI Official Symbol: STX5
• NCBI Official Synonym Symbols: SED5; STX5A
• NCBI Protein Information: syntaxin-5; syntaxin 5A
• UniProt Protein Name: Syntaxin-5
• Protein Family: Syntaxin
• UniProt Gene Name: STX5
• UniProt Synonym Gene Names: STX5A

NCBI Description

This gene encodes a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for v-SNAREs (vesicle-SNAP receptors), permitting specific synaptic vesicle docking and fusion. The encoded protein regulates endoplasmic reticulum to Golgi transport and plays a critical role in autophagy. Autoantibodies targeting the encoded protein may be a diagnostic marker for endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Mediates endoplasmic reticulum to Golgi transport. Together with p115/USO1 and GM130/GOLGA2, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus.