Western Blot (WB)
((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat STK39/SPAK Polyclonal Antibody | anti-STK39 antibody
Goat anti-STK39/SPAK Antibody
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 65kDa band observed in Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5kDa according to NP_037365.2). Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 60.3kDa according to Mouse NP_058562.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse (A) and Rat (B) Brain (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse (A) and Rat (B) Brain (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq, Jul 2008]
Uniprot Description
STLK3: May act as a mediator of stress-activated signals. The phosphorylated form forms a complex with WNK2. Predominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; FRAY subfamily
Chromosomal Location of Human Ortholog: 2q24.3
Cellular Component: apical plasma membrane; basolateral plasma membrane; cytoplasm; cytoskeleton; nucleus
Molecular Function: ATP binding; protein binding; protein kinase binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: negative regulation of protein amino acid phosphorylation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of potassium ion transport; protein amino acid phosphorylation; regulation of blood pressure; regulation of inflammatory response; response to stress
Research Articles on STK39
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Product Notes
The STK39 stk39 (Catalog #AAA421747) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-STK39/SPAK Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's STK39/SPAK can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Recommended concentration, 3-5ug/ml. Western Blot: Approx 65kDa band observed in Human Brain (Cerebellum) lysates and in lysates of cell line Jurkat (calculated MW of 59.5kDa according to NP_037365.2). Approx 75kDa band observed in Mouse and Rat Brain lysates (calculated MW of 60.3kDa according to Mouse NP_058562.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the STK39 stk39 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SQEKSRRVKE ENPE. It is sometimes possible for the material contained within the vial of "STK39/SPAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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