Rabbit anti-Human, Mouse STK35 Polyclonal Antibody | anti-STK35 antibody

STK35, ID (Serine/Threonine Kinase 35, CLIK1, CLIK-1, CLP-36-interacting Kinase 1, PDIK1, PDLIM1-interacting Kinase 1, Serine/Threonine-protein Kinase 35, Serine/Threonine-protein Kinase 35 L1, STK35L1) (Biotin)

Cat. Num. AAA6501212

• Gene Names: STK35; CLIK1; STK35L1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: STK35; Polyclonal Antibody; ID (Serine/Threonine Kinase 35; CLIK1; CLIK-1; CLP-36-interacting Kinase 1; PDIK1; PDLIM1-interacting Kinase 1; Serine/Threonine-protein Kinase 35; Serine/Threonine-protein Kinase 35 L1; STK35L1) (Biotin); anti-STK35 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STK35. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-STK35 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the central region of human STK35 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-STK35 Pab is used in Western blot to detect STK35 in mouse kidney tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(STK35 Antibody (C255) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the STK35 antibody detected the STK35 protein (arrow).)

Product Categories/Family for anti-STK35 antibody

Antibodies; Protein Kinases

References

1. Vallenius, T., et al., J. Cell. Sci. 115(Pt 10), 2067-2073 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 260099625
• NCBI GeneID: 140901
• NCBI Accession #: NP_543026
• NCBI GenBank Nucleotide #: NM_080836
• UniProt Accession #: Q8TDR2
• Molecular Weight: 44kDa
• NCBI Official Full Name: serine/threonine-protein kinase 35
• NCBI Official Synonym Full Names: serine/threonine kinase 35
• NCBI Official Symbol: STK35
• NCBI Official Synonym Symbols: CLIK1; STK35L1
• NCBI Protein Information: serine/threonine-protein kinase 35
• UniProt Protein Name: Serine/threonine-protein kinase 35
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK35
• UniProt Synonym Gene Names: CLIK1; PDIK1; STK35L1; CLIK-1
• UniProt Entry Name: STK35_HUMAN

NCBI Description

The protein encoded by this gene is a kinase that is predominantly found in the nucleus. However, it can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. The encoded protein may be a regulator of actin stress fibers in nonmuscle cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Interacts with PDLIM1/CLP-36.

Subcellular location: Nucleus. Nucleus › nucleolus. Cytoplasm. Note: When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (Ref.5). However, Ref.1 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location. Ref.1 Ref.5

Tissue specificity: Expressed in testis.

Post-translational modification: Autophosphorylated. Ref.5

Miscellaneous: Association with PDLIM1 is controversial (Ref.5).

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 protein kinase domain.

Sequence caution: The sequence AAI40884.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAI40885.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAG37270.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAI15590.1 differs from that shown. Reason: Erroneous gene model prediction.