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Western Blot (WB) (Mouse Stk3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Stk3 antibody detected the Stk3 protein (arrow).)

Rabbit Stk3 Polyclonal Antibody | anti-Stk3 antibody

Mouse Stk3 Antibody (C-term)

Gene Names
Stk3; MST; Mst2; Mst3; Ste20; mess1; 0610042I06Rik
Reactivity
Human (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Stk3; Polyclonal Antibody; Mouse Stk3 Antibody (C-term); Serine/threonine-protein kinase 3; Mammalian STE20-like protein kinase 2; MST-2; STE20-like kinase MST2; Serine/threonine-protein kinase 3 36kDa subunit; MST2/N; Serine/threonine-protein kinase 3 20kDa subunit; MST2/C; Mess1; Mst2; anti-Stk3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Stk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of mouse Stk3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
387-415
Sequence Length
427
Applicable Applications for anti-Stk3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Stk3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Stk3 antibody detected the Stk3 protein (arrow).)

Western Blot (WB) (Mouse Stk3 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the Stk3 antibody detected the Stk3 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Stk3 (arrow) using rabbit polyclonal Mouse Stk3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Stk3 gene.)

Western Blot (WB) (Western blot analysis of Stk3 (arrow) using rabbit polyclonal Mouse Stk3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Stk3 gene.)
Related Product Information for anti-Stk3 antibody
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
Product Categories/Family for anti-Stk3 antibody
References
Oh, H.J., et al. Curr. Biol. 20(5):416-422(2010)
Lu, L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1437-1442(2010)
Song, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1431-1436(2010)
Zhou, D., et al. Cancer Cell 16(5):425-438(2009)
Choi, J., et al. PLoS ONE 4 (11), E8011 (2009) :

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56855
NCBI Official Full Name
serine/threonine-protein kinase 3
NCBI Official Synonym Full Names
serine/threonine kinase 3
NCBI Official Symbol
Stk3
NCBI Official Synonym Symbols
MST; Mst2; Mst3; Ste20; mess1; 0610042I06Rik
NCBI Protein Information
serine/threonine-protein kinase 3
UniProt Protein Name
Serine/threonine-protein kinase 3
UniProt Gene Name
Stk3
UniProt Synonym Gene Names
Mess1; Mst2; MST-2; MST2/N; MST2/C
UniProt Entry Name
STK3_MOUSE

Uniprot Description

MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.

Protein type: Autophagy; Protein kinase, STE; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE20 family; MST subfamily

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; magnesium ion binding; kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: central nervous system development; protein stabilization; positive regulation of protein binding; positive regulation of apoptosis; apoptosis; positive regulation of JNK cascade; negative regulation of organ growth; signal transduction; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; neural tube formation; phosphorylation

Research Articles on Stk3

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Product Notes

The Stk3 stk3 (Catalog #AAA9204660) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 387-415. The Mouse Stk3 Antibody (C-term) reacts with Human (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Stk3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Stk3 stk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Stk3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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