Western Blot (WB)
(Mouse Stk25 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Stk25 antibody detected the Stk25 protein (arrow).)
Rabbit anti-Human Stk25 Polyclonal Antibody | anti-STK25 antibody
Stk25, CT (Stk25, Sok1, Serine/threonine-protein kinase 25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Mouse Stk25 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Stk25 antibody detected the Stk25 protein (arrow).)
Western Blot (WB)
(Mouse Stk25 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Stk25 antibody detected the Stk25 protein (arrow).)
Western Blot (WB)
(Western blot analysis of Stk25 (arrow) using rabbit polyclonal Mouse Stk25 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Stk25 gene.)
Western Blot (WB)
(Western blot analysis of Stk25 (arrow) using rabbit polyclonal Mouse Stk25 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Stk25 gene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
Uniprot Description
Function: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Ref.9
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Enzyme regulation: Interaction with Golgi matrix protein GOLGA2 leads to autophosphorylation on Thr-174, possibly as a consequence of stabilization of dimer formation. The C-terminal non-catalytic region inhibits the kinase activity. Ref.9
Subunit structure: Homodimer. Interacts with CTTNBP2NL. Ref.9 Ref.10
Subcellular location: Cytoplasm. Golgi apparatus. Note: Localizes to the Golgi apparatus. Ref.9
Tissue specificity: Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung.
Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 protein kinase domain.
Research Articles on STK25
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Product Notes
The STK25 stk25 (Catalog #AAA645948) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Stk25, CT (Stk25, Sok1, Serine/threonine-protein kinase 25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Stk25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the STK25 stk25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Stk25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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