Western Blot (WB)
(Western blot analysis of hSTEP-Q155 in mouse brain tissue lysates (35ug/lane). STEP (arrow) was detected using the purified Pab)
Rabbit anti-Human, mouse STEP / PTPN5 Polyclonal Antibody | anti-PTPN5 antibody
STEP / PTPN5 Antibody (N-Term)
Gene Names
PTPN5; STEP; PTPSTEP
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
STEP / PTPN5; Polyclonal Antibody; STEP / PTPN5 Antibody (N-Term); Tyrosine-protein phosphatase non-receptor type 5; Neural-specific protein-tyrosine phosphatase; Striatum-enriched protein-tyrosine phosphatase; STEP; PTPN5; anti-PTPN5 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This STEP / PTPN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the N-terminal region of human STEP / PTPN5.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
168-198
Sequence Length
541
Applicable Applications for anti-PTPN5 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
IHC-P~~1:10~50
IHC-P~~1:10~50
Function
May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
Cellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of hSTEP-Q155 in mouse brain tissue lysates (35ug/lane). STEP (arrow) was detected using the purified Pab)
Western Blot (WB)
(Western blot analysis of hSTEP-Q155 in mouse brain tissue lysates (35ug/lane). STEP (arrow) was detected using the purified Pab)
Western Blot (WB)
(Western blot analysis of hSTEP-Q155 in HepG2 cell line lysates (35ug/lane). STEP (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of hSTEP-Q155 in HepG2 cell line lysates (35ug/lane). STEP (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with STEP antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with STEP antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-PTPN5 antibody
STEP (striatum-enriched phosphatase) is a neural-specific protein-tyrosine phosphatase, first isolated from the rat brain. The 537-amino acid predicted human protein as isolated from cDNA sequences is between 85 and 90% identical to the mouse and rat sequences. In rat neuronal cell cultures, glutamate-mediated activation of N-methyl-D-aspartate (NMDA) receptors leads to the rapid but transient phosphorylation of extracellular signal-related kinase-2 (ERK2). NMDA-mediated influx of calcium, activates the calcium-dependent phosphatase calcineurin and the resulting dephosphorylation and activation of STEP. STEP then inactivatea ERK2 through tyrosine dephosphorylation and blocks translocation of the kinase to the nucleus. STEP plays a significant role in regulating the ERK activation and downstream signaling in neurons.
Product Categories/Family for anti-PTPN5 antibody
References
Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
Li, X., et al., Genomics 28(3):442-449 (1995).
Li, X., et al., Genomics 28(3):442-449 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63538
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 5 isoform b
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
NCBI Official Symbol
PTPN5
NCBI Official Synonym Symbols
STEP; PTPSTEP
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 5
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 5
Protein Family
UniProt Gene Name
PTPN5
UniProt Synonym Gene Names
STEP
UniProt Entry Name
PTN5_HUMAN
Uniprot Description
STEP: a non-receptor protein-tyrosine phosphatase. STEP (striatum enriched phosphatase) Contains a single phosphatase catalytic domain located at the C-terminus. Two alternatively-spliced messages have been described.
Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Protein phosphatase, tyrosine (non-receptor)
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: phosphotyrosine binding; protein tyrosine phosphatase activity
Biological Process: protein amino acid dephosphorylation
Research Articles on PTPN5
Similar Products
Product Notes
The PTPN5 ptpn5 (Catalog #AAA9204706) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 168-198. The STEP / PTPN5 Antibody (N-Term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STEP / PTPN5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the PTPN5 ptpn5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STEP / PTPN5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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