Rabbit Stella Polyclonal Antibody | anti-DPPA3 antibody

Stella Antibody

Cat. Num. AAA9402500

• Gene Names: DPPA3; STELLA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: Stella; Polyclonal Antibody; Stella Antibody; Development pluripotency-associated 3; DPPA3; primordial germ cell 7; PGC7; anti-DPPA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 159

• Applicable Applications for anti-DPPA3 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 13 amino acid peptide from near the carboxy terminus of human Stella.
• Target Name: Stella
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Stella in 293 cell lysate with Stella antibody at (A) 1 and (B) 2 ug/mL.)

Related Product Information for anti-DPPA3 antibody

Stella was initially identified in primordial germ cells and pre-implantation embryos whose expression as a maternal factor is important in early embryonic development but is not required for germ cell specification in mice. In humans, Stella is thought to be a marker for pluripotency in embryonic stem (ES) cells as its expression is observed in primordial germ cells of both sexes and germ cell tumors but not in normal somatic tissues. However, in ES cell colonies, heterogeneous expression of Stella was seen in high throughput in situ hybridization assays, indicating that higher levels of complexity exist in otherwise thought to be undifferentiated ES cells. At least two distinct isoforms of Stella are known to exist.

Product Categories/Family for anti-DPPA3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 40548326
• NCBI GeneID: 359787
• NCBI Accession #: NP_954980
• NCBI GenBank Nucleotide #: NM_199286.3
• Molecular Weight: 17,851 Da
• NCBI Official Full Name: developmental pluripotency-associated protein 3
• NCBI Official Synonym Full Names: developmental pluripotency associated 3
• NCBI Official Symbol: DPPA3
• NCBI Official Synonym Symbols: STELLA
• NCBI Protein Information: developmental pluripotency-associated protein 3
• UniProt Protein Name: Developmental pluripotency-associated protein 3
• Protein Family: Stellacyanin
• UniProt Gene Name: DPPA3
• UniProt Synonym Gene Names: STELLAR
• UniProt Entry Name: DPPA3_HUMAN

NCBI Description

This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008]

Uniprot Description

DPPA3: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: male pronucleus; cytoplasm; female pronucleus; nucleus

Molecular Function: methylated histone residue binding

Biological Process: chromatin modification; embryonic cleavage